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Legionella

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Carmen Buchrieser

    1. , Biology of Intracellular Bacteria, Institut Pasteur, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Hubert Hilbi

    1. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Munich, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 954)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xxviii
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  2. Identification and Typing of Legionella in the Environment and During Disease

    1. Front Matter

      Pages 1-1
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    2. Identification of Legionella in the Environment

      • Natalia A. Kozak, Claressa E. Lucas, Jonas M. Winchell
      Pages 3-25

    3. Identification of Legionella in Clinical Samples

      • Sophie Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, Jerome Etienne
      Pages 27-56

    4. Sequence-Based Identification of Legionella

      • Rodney M. Ratcliff
      Pages 57-72

    5. Investigation of Outbreaks: Epidemiology

      • Carol Joseph
      Pages 73-86

    6. Outbreak Investigations and Identification of Legionella in Contaminated Water

      • Susanne Lee, John Lee
      Pages 87-118

    7. Typing Methods for Legionella

      • Christian Lück, Norman K. Fry, Jürgen H. Helbig, Sophie Jarraud, Timothy G. Harrison
      Pages 119-148

  3. Physiology and Genetics of Legionella

    1. Front Matter

      Pages 149-149
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  4. Physiology and genetics of Legionella

    1. Culturing, Media, and Handling of Legionella

      • Christa H. Chatfield, Nicholas P. Cianciotto
      Pages 151-162

    2. The Intracellular Metabolism of Legionella by Isotopologue Profiling

      • Klaus Heuner, Wolfgang Eisenreich
      Pages 163-181

    3. Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction

      • Carmen Buchrieser, Xavier Charpentier
      Pages 183-195

    4. Constructing Unmarked Gene Deletions in Legionella pneumophila

      • Andrew Bryan, Zachary D. Abbott, Michele S. Swanson
      Pages 197-212

    5. Natural Biofilm Formation with Legionella pneumophila

      • Emilie Portier, Yann Héchard
      Pages 213-217

    6. Production of L. pneumophila Monospecies Biofilms in a Low-Nutrient-Concentration Medium

      • Sophie Pécastaings, Christine Roques
      Pages 219-224

    7. Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila

      • Jens Jäger, Michael Steinert
      Pages 225-230

  5. Cellular Microbiology of Legionella

    1. Front Matter

      Pages 231-231
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  6. Cellular microbiology of Legionella

    1. Analysis of Legionella Infection by Flow Cytometry

      • André N. Tiaden, Aline Kessler, Hubert Hilbi
      Pages 233-249

    2. Analysis of Legionella Infection Using RNAi in Drosophila Cells

      • Dennise A. De Jesús, Tamara J. O’Connor, Ralph R. Isberg
      Pages 251-264

    3. Immunofluorescence Imaging of Legionella Infection In Vitro

      • Stefan S. Weber, Annette Oxenius
      Pages 265-277
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Keywords

About this book

Legionnaires’ disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of Legionella spp. will contribute to an efficient and sustainable elimination of the bacteria from anthropogenic water systems and might lead to the development of novel therapeutics. The many different methods described in Legionella: Methods and Protocols are all specifically adapted and developed for the study of distinct features of L. pneumophila, and have already led to exciting discoveries. Most of these techniques can also be applied to the study of other bacterial pathogens, in particular intracellular pathogens like Shigella, Salmonella or Listeria. .  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Legionella: Methods and Protocols will not only be useful for research groups studying Legionella, but also for a broader scientific community studying the epidemiology, typing, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.

Editors and Affiliations

  • , Biology of Intracellular Bacteria, Institut Pasteur, Paris, France

    Carmen Buchrieser

  • Max von Pettenkofer Institute, Ludwig-Maximilians-University, Munich, Germany

    Hubert Hilbi

Bibliographic Information

  • Book Title: Legionella

  • Book Subtitle: Methods and Protocols

  • Editors: Carmen Buchrieser, Hubert Hilbi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-161-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-160-8Published: 13 November 2012

  • Softcover ISBN: 978-1-4939-6274-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-161-5Published: 13 November 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXVIII, 622

  • Topics: Infectious Diseases, Water and Health, Bacteriology

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