Single Domain Antibodies

1. Front Matter

   Pages i-xv

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2. Overview of Single Domain Antibodies

   1. Front Matter

      Pages 1-1

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   2. From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

      • Jean-Luc Teillaud

      Pages 3-13

   3. Introduction to Heavy Chain Antibodies and Derived Nanobodies

      • Cécile Vincke, Serge Muyldermans

      Pages 15-26

   4. Overview and Discovery of IgNARs and Generation of VNARs

      • Stewart D. Nuttall

      Pages 27-36

3. Single Domain Antibody Library Construction

   1. Front Matter

      Pages 37-37

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   2. Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

      • Olga Ignatovich, Laurent Jespers, Ian M. Tomlinson, Ruud M. T. de Wildt

      Pages 39-63

   3. Preparation of a Naïve Library of Camelid Single Domain Antibodies

      • Aurelien Olichon, Ario de Marco

      Pages 65-78

4. Selection of Single Domain Antibodies

   1. Front Matter

      Pages 79-79

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   2. Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation

      • Peter Verheesen, Toon Laeremans

      Pages 81-104

   3. Semiautomated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens

      • Jyothi Kumaran, C. Roger MacKenzie, Mehdi Arbabi-Ghahroudi

      Pages 105-124

   4. Pichia Surface Display: A Tool for Screening Single Domain Antibodies

      • Kristof De Schutter, Nico Callewaert

      Pages 125-134

   5. Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method

      • Mireille Pellis, Serge Muyldermans, Cécile Vincke

      Pages 135-150

   6. Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

      • Tomoyuki Tanaka, Terence H. Rabbitts

      Pages 151-173

   7. Selection of Functional Single Domain Antibody Fragments for Interfering with Protein–Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System

      • Tomoyuki Tanaka, Terence H. Rabbitts

      Pages 175-182

   8. Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization

      • Armin Sepp, Andrew Griffiths

      Pages 183-198

   9. Selection of VHHs Under Application Conditions

      • Edward Dolk, Theo Verrips, Hans de Haard

      Pages 199-209

   10. Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies

       • Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, Jamshid Tanha

       Pages 211-239

   11. Selection of VHH Antibody Fragments That Recognize Different Aβ Depositions Using Complex Immune Libraries

       • Rinse Klooster, Kim S. Rutgers, Silvère M. van der Maarel

       Pages 241-253

5. Expression of Single Domain Antibodies and Derivatives

   1. Front Matter

      Pages 225-225

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The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

• Heavy chain antibodies
• Single variable domain
• V-NAR
• VHH
• sdAbs
• antibodies

• , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium

  Dirk Saerens, Serge Muyldermans

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