Editors:
- Details many of the methods currently used to study RNA silencing in relation to viral infections in plants
- Features step-by-step, easy to follow protocols from an international assortment of laboratories
- Includes key tips and expert implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 894)
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Table of contents (24 protocols)
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Front Matter
About this book
Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.Editors and Affiliations
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Division of Plant Industry, CSIRO, Canberra, Australia
John M. Watson, Ming-Bo Wang
Bibliographic Information
Book Title: Antiviral Resistance in Plants
Book Subtitle: Methods and Protocols
Editors: John M. Watson, Ming-Bo Wang
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-882-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-881-8Published: 12 June 2012
Softcover ISBN: 978-1-4939-5722-4Published: 23 August 2016
eBook ISBN: 978-1-61779-882-5Published: 08 June 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 380
Number of Illustrations: 49 b/w illustrations, 10 illustrations in colour
Topics: Plant Sciences, Plant Pathology, Virology