Overview
- Focuses on mouse models but features other popular models as well
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 886)
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Table of contents (36 protocols)
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Front Matter
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Organ Culture, Tissue Manipulation, and Live Imaging
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Front Matter
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Analyzing Ureteric Bud Branching and Nephrogenesis
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Front Matter
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Renal Primary Cell Cultures and Cell Lines
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Front Matter
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Keywords
About this book
Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds. One fundamental aspect of development biology is the concept of morphogenesis: understanding how a group of multipotent cells organize and differentiate into a complex organ. In Kidney Development: Methods and Protocols, expert researchers in the field detail different approaches to tackle kidney development. These approaches include culture and live imaging aspects of kidney development, analyzing the 3-dimensional aspects of branching morphogenesis as well as nephrogenesis, manipulation of the gene/protein expression during kidney development as well as in the adult kidney, and how to assess kidney malformation and disease. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Kidney Development: Methods and Protocols seeks to aid scientists in the further study of the process of morphogenesis which is fundamental important not only for studying developmental biology but also for regenerative medicine.
Editors and Affiliations
Bibliographic Information
Book Title: Kidney Development
Book Subtitle: Methods and Protocols
Editors: Odyssé Michos
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-851-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-850-4Published: 27 May 2012
Softcover ISBN: 978-1-4939-6255-6Published: 23 August 2016
eBook ISBN: 978-1-61779-851-1Published: 26 May 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 415
Topics: Molecular Medicine, Nephrology