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  • © 2012

Nanopore-Based Technology

  • Provides a good representation of present-day available techniques for biomolecule characterization with nanoporous membranes
  • Includes many step-by-step, easy to follow protocols
  • Features tips from the experts to ensure successful implementation
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 870)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xi
  2. Single Molecule Characterization with Biological Nanopores

    1. Front Matter

      Pages 1-1
    2. Detecting and Characterizing Individual Molecules with Single Nanopores

      • John J. Kasianowicz, Joseph E. Reiner, Joseph W. F. Robertson, Sarah E. Henrickson, Claudio Rodrigues, Oleg V. Krasilnikov
      Pages 3-20
    3. Protein Sensing with Engineered Protein Nanopores

      • Mohammad M. Mohammad, Liviu Movileanu
      Pages 21-37
    4. Measurements of DNA Immobilized in the Alpha-Hemolysin Nanopore

      • Robert Purnell, Jacob Schmidt
      Pages 39-53
    5. DNA Unzipping and Protein Unfolding Using Nanopores

      • Céline Merstorf, Benjamin Cressiot, Manuela Pastoriza-Gallego, Abdel Ghani Oukhaled, Laurent Bacri, Jacques Gierak et al.
      Pages 55-75
  3. Biomolecule Characterization with Artificial Membranes

    1. Front Matter

      Pages 77-77
  4. Bio-Molecule Characterization with Artificial Membranes

    1. DNA Characterization with Ion Beam-Sculpted Silicon Nitride Nanopores

      • Ryan C. Rollings, David S. McNabb, Jiali Li
      Pages 79-97
    2. DNA Sequencing by Nanopore-Induced Photon Emission

      • Alon Singer, Ben McNally, Ruby Dela Torre, Amit Meller
      Pages 99-114
    3. Analyzing Single DNA Molecules by Nanopore Translocation

      • Lorenz J. Steinbock, Ulrich F. Keyser
      Pages 135-145
  5. Theory and Computer Simulations for Single Molecule Characterization with Protein and Solid-State Nanochannels

    1. Front Matter

      Pages 147-147
    2. DNA Characterization by Transverse Electrical Current in a Nanochannel

      • Massimiliano Di Ventra, Matt Krems, James Wilson, Yuriy V. Pershin
      Pages 149-163
    3. Optimization of the Molecular Dynamics Method for Simulations of DNA and Ion Transport Through Biological Nanopores

      • David B. Wells, Swati Bhattacharya, Rogan Carr, Christopher Maffeo, Anthony Ho, Jeffrey Comer et al.
      Pages 165-186
    4. Polymer Translocation Through an Electrically Tunable Nanopore in a Multilayered Semiconductor Membrane

      • Dmitriy V. Melnikov, Alexey Nikolaev, Jean-Pierre Leburton, Maria E. Gracheva
      Pages 187-207
  6. Novel Materials and Devices for Biomolecular Characterization

    1. Front Matter

      Pages 209-209
  7. Novel Materials and Devices for Bio-Molecular Characterization

    1. Graphene Nanopore Devices for DNA Sensing

      • Chris A. Merchant, Marija Drndić
      Pages 211-226
    2. Measuring Single-Wall Carbon Nanotubes with Solid-State Nanopores

      • Adam R. Hall, Johannes M. Keegstra, Matthew C. Duch, Mark C. Hersam, Cees Dekker
      Pages 227-239
    3. Passive and Electrically Actuated Solid-State Nanopores for Sensing and Manipulating DNA

      • Zhijun Jiang, Mirna Mihovilovic, Erin Teich, Derek Stein
      Pages 241-264
  8. Back Matter

    Pages 265-267

About this book

Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality.  In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization.  Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing.  Written for the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results.

 

Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.

Editors and Affiliations

  • , Department of Physics, Clarkson University, Potsdam, USA

    Maria E. Gracheva

Bibliographic Information

  • Book Title: Nanopore-Based Technology

  • Editors: Maria E. Gracheva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-773-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-772-9Published: 18 April 2012

  • Softcover ISBN: 978-1-4939-5809-2Published: 23 August 2016

  • eBook ISBN: 978-1-61779-773-6Published: 23 April 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 267

  • Topics: Biotechnology, Nanotechnology

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access