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  • © 2009

Molecular Endocrinology

Methods and Protocols

  • Provides an easily accessible reference volume on techniques in endocrinology written by leading researchers in the field
  • Presents step-by-step instructions for each technique to insure successful results
  • Includes tricks of the trade and notes on troubleshooting presented to avoid known pitfalls
  • Covers a wide range of cutting-edge methods in endocrinology
  • Comprehensive guide to performing state of the art protocols in endocrinology
  • Details the process of setting up, running, and analyzing a wide variety of endocrinology based techniques
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 590)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
  2. Determining the Affinity of Hormone−Receptor Interaction

    • David Puett, Krassimira Angelova
    Pages 1-20
  3. Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques

    • Carole Bryant, John Moore, Thomas E. Curry Jr
    Pages 21-32
  4. Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane

    • Mary Osisami, Huiyan Huang, Michael A. Frohman
    Pages 61-70
  5. Gene Expression Profiling in the Aging Ovary

    • Kathleen M. Eyster, John D. Brannian
    Pages 71-89
  6. Genomics Analysis: Endometrium

    • Ricardo Francalacci Savaris, Linda C. Giudice
    Pages 91-113
  7. Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization

    • Katherine L. Rosewell, Thomas E. Curry Jr
    Pages 115-129
  8. Adenoviral Gene Transfer into Isolated Pancreatic Islets

    • Latha Muniappan, Sabire Özcan
    Pages 131-142
  9. Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor α

    • Jennifer R. Schultz-Norton, Yvonne S. Ziegler, Varsha S. Likhite, Ann M. Nardulli
    Pages 209-221
  10. Chromosome-Wide Analysis of Protein Binding and Modifications

    • Kevin D. Sarge, Hongyan Xing, Ok-Kyong Park-Sarge
    Pages 223-233
  11. Detection of ERα-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer

    • Tamika T Duplessis, Kristen L. Koterba, Brian G. Rowan
    Pages 253-263
  12. Detection of Proteins Sumoylated In Vivo and In Vitro

    • Kevin D. Sarge, Ok-Kyong Park-Sarge
    Pages 265-277
  13. Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE)

    • Oladapo Yeku, Elizabeth Scotto-Lavino, Michael A. Frohman
    Pages 279-294
  14. Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis

    • Sachiko Matsuzaki, Michel Canis, Gérard Mage
    Pages 295-306

About this book

Over the course of the last hundred years, the field of endocrinology has received ever-increasing attention. Research and developing technologies have resulted in a significant leap in our understanding of the causes and treatment of endocrine dysfunction. In Molecular Endocrinology: Methods and Protocols, leaders in the field share a diversity of cutting-edge techniques that are becoming routinely employed in the quest to further understand hormone action.  In order to reach the widest number of labs, this volume includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and easy to use, Molecular Endocrinology: Methods and Protocols is an ideal guide for researchers in labs across the world who seek a deeper understanding of the complex processes and biology of hormones.

Editors and Affiliations

  • College of Medicine, University of Kentucky, Lexington, U.S.A.

    Ok-Kyong Park-Sarge

  • Dept. Obstetrics & Gynecology, University of Kentucky, Lexington, U.S.A.

    Thomas E. Curry

Bibliographic Information

  • Book Title: Molecular Endocrinology

  • Book Subtitle: Methods and Protocols

  • Editors: Ok-Kyong Park-Sarge, Thomas E. Curry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-378-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-377-0Published: 04 December 2009

  • Softcover ISBN: 978-1-61779-668-5Published: 25 February 2012

  • eBook ISBN: 978-1-60327-378-7Published: 18 September 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 430

  • Number of Illustrations: 98 b/w illustrations, 1 illustrations in colour

  • Topics: Endocrinology, Human Physiology, Molecular Medicine

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access