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Mobile Genetic Elements

Protocols and Genomic Applications

  • Book
  • © 2012

Overview

Editors:
  1. Yves Bigot

    1. , Physiologie de la Reproduction, UMR INRA-CNRS 6175, Nouzilly Cedex, France

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  • Describes TEs as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 859)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Transposable Elements as Tools for Reshaping the Genome: It Is a Huge World After All!

    • Solenne Bire, Florence Rouleux-Bonnin
    Pages 1-28

  3. Using and Understanding RepeatMasker

    • Sébastien Tempel
    Pages 29-51

  4. Roadmap for Annotating Transposable Elements in Eukaryote Genomes

    • Emmanuelle Permal, Timothée Flutre, Hadi Quesneville
    Pages 53-68

  5. To Detect and Analyze Sequence Repeats Whatever Be Their Origin

    • Jacques Nicolas
    Pages 69-90

  6. Exploring Bacterial Insertion Sequences with ISfinder: Objectives, Uses, and Future Developments

    • P. Siguier, A. Varani, J. Perochon, M. Chandler
    Pages 91-103

  7. Methods and Software in NGS for TE Analysis

    • Cristian Chaparro, Francois Sabot
    Pages 105-114

  8. The Application of LTR Retrotransposons as Molecular Markers in Plants

    • Alan H. Schulman, Andrew J. Flavell, Etienne Paux, T. H. Noel Ellis
    Pages 115-153

  9. Individual Analysis of Transposon Polymorphisms by AFLP

    • Susanta K. Behura
    Pages 155-167

  10. Construction of a Library of Random Mutants in the Spirochete Leptospira Biflexa Using a Mariner Transposon

    • Leyla Slamti, Mathieu Picardeau
    Pages 169-176

  11. Ac–Ds Solutions for Rice Insertion Mutagenesis

    • Emmanuel Guiderdoni, Pascal Gantet
    Pages 177-187

  12. Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion

    • Valérie J. P. Robert
    Pages 189-201

  13. Genome-Wide Manipulations of Drosophila melanogaster with Transposons, Flp Recombinase, and ΦC31 Integrase

    • Koen J. T. Venken, Hugo J. Bellen
    Pages 203-228

  14. The Sleeping Beauty Transposon Toolbox

    • Ismahen Ammar, Zsuzsanna Izsvák, Zoltán Ivics
    Pages 229-240

  15. PiggyBac Toolbox

    • Mario Di Matteo, Janka Mátrai, Eyayu Belay, Tewodros Firdissa, Thierry VandenDriessche, Marinee K. L. Chuah
    Pages 241-254

  16. Insertion Site Pattern: Global Approach by Linear Amplification-Mediated PCR and Mass Sequencing

    • Cynthia C. Bartholomae, Hanno Glimm, Christof von Kalle, Manfred Schmidt
    Pages 255-265

  17. Comprehensive DNA Methylation Profiling of Human Repetitive DNA Elements Using an MeDIP-on-RepArray Assay

    • Eric Gilson, Béatrice Horard
    Pages 267-291

  18. Novel Approach for the Development of New Antibodies Directed Against Transposase-Derived Proteins Encoded by Human Neogenes

    • Ahmed Arnaoty, Bruno Pitard, Benoit Bateau, Yves Bigot, Thierry Lecomte
    Pages 293-305

  19. Back Matter

    Pages 307-308
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Keywords

About this book

Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism. In, Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition gathers a wide array of strategies and protocols for identifying transposable elements (TEs) and their evolutionary derivatives, and for studying genome structure, function, and evolution. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Through and intuitive Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition aids scientists in the continuing study of in silico tools, strategies and protocols that are complementary of those described in the first volume.

Editors and Affiliations

  • , Physiologie de la Reproduction, UMR INRA-CNRS 6175, Nouzilly Cedex, France

    Yves Bigot

Bibliographic Information

  • Book Title: Mobile Genetic Elements

  • Book Subtitle: Protocols and Genomic Applications

  • Editors: Yves Bigot

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-603-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-602-9Published: 25 February 2012

  • Softcover ISBN: 978-1-4939-5923-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-603-6Published: 25 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 308

  • Topics: Human Genetics

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