Overview
- Examines the culture and genetic manipulation of primary fungal pathogens as well as interactions in model systems
- Includes detailed, tested methods that are ready for the lab
- Features key tips and implementation advice necessary to achieve successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 845)
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Table of contents (42 protocols)
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Front Matter
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Gene Disruption
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Front Matter
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Modulation of Gene Expression: RNAi Gene Knockdown
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Front Matter
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Modulation of Gene Expression: Regulatable Promoters
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Front Matter
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Host Responses to Infection In Vitro
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Front Matter
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Keywords
About this book
Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease. Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.
Reviews
From the book reviews:
“The book ‘Host-fungus Interactions’ assembles methods and protocols for studying gene disruption, modulation of gene expression, host and fungal responses to infection and several model systems to analyze the host-fungus interactions. The book comprises of 8 parts with 42 chapters and an index at the end of the book. … A useful tool for medical microbiologists, immunologists, geneticists, biotechnologists and students studying pharmacology and pathogenicity testing.” (Melvina D’souza and Kevin D. Hyde, Fungal Diversity, December, 2014)Editors and Affiliations
Bibliographic Information
Book Title: Host-Fungus Interactions
Book Subtitle: Methods and Protocols
Editors: Alexandra C. Brand, Donna M. MacCallum
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-539-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-538-1Published: 10 February 2012
Softcover ISBN: 978-1-4939-5904-4Published: 23 August 2016
eBook ISBN: 978-1-61779-539-8Published: 11 February 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 597
Number of Illustrations: 67 b/w illustrations, 37 illustrations in colour
Topics: Microbiology, Pathology, Laboratory Medicine