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  • © 2012

Mitochondrial Bioenergetics

Methods and Protocols

  • Elevates the quality and rate of investigative discoveries regarding disease state associated with enviromental or genetic influences on mitochondrial bioenergentics
  • Provides step-by-step detail essentail for reproducible results
  • Contains key notes and implementaion advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 810)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Overview of Mitochondrial Bioenergetics

    • Vitor M. C. Madeira
    Pages 1-6
  3. High-Throughput Analysis of Mitochondrial Oxygen Consumption

    • James Hynes, Rachel L. Swiss, Yvonne Will
    Pages 59-72
  4. Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells

    • Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi, Roger F. Castilho
    Pages 103-117
  5. Measurement of Proton Leak and Electron Leak in Isolated Mitochondria

    • Charles Affourtit, Casey L. Quinlan, Martin D. Brand
    Pages 165-182
  6. Relation Between Mitochondrial Membrane Potential and ROS Formation

    • Jan M. Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, Mariusz R. Wieckowski
    Pages 183-205
  7. Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport

    • António J. M. Moreno, Joaquim A. Vicente
    Pages 207-217
  8. Mitochondrial Permeability Transition Pore and Calcium Handling

    • Renee Wong, Charles Steenbergen, Elizabeth Murphy
    Pages 235-242
  9. Imaging of Mitochondrial pH Using SNARF-1

    • Venkat K. Ramshesh, John J. Lemasters
    Pages 243-248
  10. Redox Equivalents and Mitochondrial Bioenergetics

    • James R. Roede, Young-Mi Go, Dean P. Jones
    Pages 249-280
  11. NMR Methodologies for Studying Mitochondrial Bioenergetics

    • Tiago C. Alves, Ivana Jarak, Rui A. Carvalho
    Pages 281-309
  12. Computational Modeling of Mitochondrial Function

    • Sonia Cortassa, Miguel A. Aon
    Pages 311-326

About this book

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.  These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

 

 

Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.

Editors and Affiliations

  • Center for Neurosciences and Cell Biolog, Universidad de Coimbra, Coimbra, Portugal

    Carlos M. Palmeira

  • Centro de Neurociencias, Universidad de Coimbra, Coimbra, Portugal

    António J. Moreno

Bibliographic Information

  • Book Title: Mitochondrial Bioenergetics

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos M. Palmeira, António J. Moreno

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-382-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science&Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-381-3Published: 05 November 2011

  • Softcover ISBN: 978-1-4939-5837-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-382-0Published: 06 November 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 333

  • Number of Illustrations: 58 b/w illustrations, 25 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access