Overview
- Extends and expands upon the hands-on methodologies of the popular first edition
- Features expert techniques ready for the laboratory
- Includes key tips and implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 792)
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Table of contents (19 protocols)
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Front Matter
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Setting and Performing ELISPOT Assay
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Front Matter
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Advanced ELISPOT Techniques
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Front Matter
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Image and Data Analysis
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Front Matter
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Keywords
About this book
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.
Reviews
From the reviews of the second edition:
“In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple.” (Hayley Evans, Immunology News, November, 2012)Editors and Affiliations
Bibliographic Information
Book Title: Handbook of ELISPOT
Book Subtitle: Methods and Protocols
Editors: Alexander E. Kalyuzhny
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-325-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-324-0Published: 29 September 2011
Softcover ISBN: 978-1-4939-6111-5Published: 23 August 2016
eBook ISBN: 978-1-61779-325-7Published: 30 September 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 261
Number of Illustrations: 46 b/w illustrations, 13 illustrations in colour
Topics: Immunology, Laboratory Medicine