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  • Book
  • © 2011

Nanoproteomics

Methods and Protocols

  • Attempts to standardize and proliferate these key procedures in order to lead to wide scale adoption
  • Contains easy-to-follow methods and protocols, ready for the lab
  • Features expert tips and implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 790)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
  2. Preliminary Sample Preparation

    1. Front Matter

      Pages 1-1
    2. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling

      • Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, Rainer Bischoff
      Pages 17-28
  3. Nanoscale Separation

    1. Front Matter

      Pages 29-29
    2. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces

      • Rui Vitorino, Jana Krenkova, Frantisek Foret, Pedro Domingues, Francisco Amado
      Pages 31-46
    3. Nanoelectrospray-MSn of Native and Permethylated Glycans

      • Christina Bleckmann, Hildegard Geyer, Rudolf Geyer
      Pages 71-85
    4. N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry

      • Michael S. Bereman, David C. Muddiman
      Pages 87-97
    5. Nano LC–MS/MS: A Robust Setup for Proteomic Analysis

      • Marco Gaspari, Giovanni Cuda
      Pages 115-126
  4. Nanomaterials: Nanostructured Surfaces

    1. Front Matter

      Pages 165-165
    2. Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry

      • Federico Torta, Matteo Fusi, Carlo S. Casari, Andrea Li Bassi, Angela Bachi
      Pages 173-181
    3. Protein Nanoarrays for High-Resolution Patterning of Bacteria on Gold Surfaces

      • Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, Paula M. Mendes
      Pages 191-200
  5. Nanomaterials: Nanoscale Tools for Proteomic Discovery

    1. Front Matter

      Pages 201-201

About this book

As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues.  The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.

 

Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.

Editors and Affiliations

  • , Department of Neurosurgery, Geisinger Health Systems, Danville, USA

    Steven A. Toms

  • Neurological Institute, Brain Tumor & Neuro-Oncology Center, The Cleveland Clinic, Cleveland, USA

    Robert J. Weil

Bibliographic Information

  • Book Title: Nanoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Steven A. Toms, Robert J. Weil

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-319-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-318-9Published: 23 September 2011

  • Softcover ISBN: 978-1-4939-5799-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-319-6Published: 27 October 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 322

  • Number of Illustrations: 34 b/w illustrations, 34 illustrations in colour

  • Topics: Protein Science, Proteomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access