Overview
- Provides a comprehensive catalogue of techniques for the study of cell migration
- Includes step-by-step, readily reproducible protocols
- Features key tips and expert implementation advice for successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 769)
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Table of contents (30 protocols)
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Front Matter
Keywords
About this book
Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for anyresearcher who wishes to explore this significant area of cell biology.
Editors and Affiliations
Bibliographic Information
Book Title: Cell Migration
Book Subtitle: Developmental Methods and Protocols
Editors: Claire M. Wells, Maddy Parsons
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-207-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-206-9Published: 15 July 2011
Softcover ISBN: 978-1-4939-6193-1Published: 04 May 2017
eBook ISBN: 978-1-61779-207-6Published: 15 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 463
Number of Illustrations: 71 b/w illustrations, 35 illustrations in colour
Topics: Cell Biology, Developmental Biology