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Gel-Free Proteomics

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Kris Gevaert

    1. , VIB Department of Medical Protein Resear, VIB and Ghent University, Gent, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Joël Vandekerckhove

    1. , VIB Department of Medical Protein Resear, VIB and Ghent University, Gent, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 753)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Mass Spectrometry-Driven Proteomics: An Introduction

    • Kenny Helsens, Lennart Martens, Joël Vandekerckhove, Kris Gevaert
    Pages 1-27

  3. Metabolic Labeling of Model Organisms Using Heavy Nitrogen (15N)

    • Joost W. Gouw, Bastiaan B.J. Tops, Jeroen Krijgsveld
    Pages 29-42

  4. Trypsin-Catalyzed Oxygen-18 Labeling for Quantitative Proteomics

    • Wei-Jun Qian, Brianne O. Petritis, Carrie D. Nicora, Richard D. Smith
    Pages 43-54

  5. ICPL Labeling Strategies for Proteome Research

    • Friedrich Lottspeich, Josef Kellermann
    Pages 55-64

  6. Quantitative Proteome Analysis Using Isobaric Peptide Termini Labeling (IPTL)

    • Magnus Ø. Arntzen, Christian J. Koehler, Achim Treumann, Bernd Thiede
    Pages 65-76

  7. Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins

    • Casey J. Krusemark, Brian L. Frey, Lloyd M. Smith, Peter J. Belshaw
    Pages 77-91

  8. Production and Use of Stable Isotope-Labeled Proteins for Absolute Quantitative Proteomics

    • Dorothée Lebert, Alain Dupuis, Jérôme Garin, Christophe Bruley, Virginie Brun
    Pages 93-115

  9. Organelle Proteomics

    • Sophie Duclos, Michel Desjardins
    Pages 117-128

  10. Membrane Protein Digestion – Comparison of LPI HexaLane with Traditional Techniques

    • Ping Sui, Tasso Miliotis, Max Davidson, Roger Karlsson, Anders Karlsson
    Pages 129-142

  11. GeLCMS for In-Depth Protein Characterization and Advanced Analysis of Proteomes

    • Alicia Lundby, Jesper V. Olsen
    Pages 143-155

  12. Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide-Centric MS-Driven Proteomics

    • Nadia Taouatas, Shabaz Mohammed, Albert J.R. Heck
    Pages 157-167

  13. Quantitation of Newly Synthesized Proteins by Pulse Labeling with Azidohomoalanine

    • Gertjan Kramer, Piotr T. Kasper, Luitzen de Jong, Chris G. de Koster
    Pages 169-181

  14. Analytical Strategies in Mass Spectrometry-Based Phosphoproteomics

    • Heidi Rosenqvist, Juanying Ye, Ole N. Jensen
    Pages 183-213

  15. A Protocol on the Use of Titanium Dioxide Chromatography for Phosphoproteomics

    • Martijn W. H. Pinkse, Simone Lemeer, Albert J. R. Heck
    Pages 215-228

  16. Positional Proteomics at the N-Terminus as a Means of Proteome Simplification

    • Gemma R. Davidson, Stuart D. Armstrong, Robert J. Beynon
    Pages 229-242

  17. N-Terminomics: A High-Content Screen for Protease Substrates and Their Cleavage Sites

    • John C. Timmer, Guy S. Salvesen
    Pages 243-255

  18. Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-Derived Peptide Libraries

    • Oliver Schilling, Ulrich auf dem Keller, Christopher M. Overall
    Pages 257-272

  19. Identification of Proteolytic Products and Natural Protein N-Termini by Terminal Amine Isotopic Labeling of Substrates (TAILS)

    • Alain Doucet, Oded Kleifeld, Jayachandran N. Kizhakkedathu, Christopher M. Overall
    Pages 273-287

  20. Lectins as Tools to Select for Glycosylated Proteins

    • Els J. M. Van Damme
    Pages 289-297
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Keywords

About this book

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursing research in this critical field.

Editors and Affiliations

  • , VIB Department of Medical Protein Resear, VIB and Ghent University, Gent, Belgium

    Kris Gevaert, Joël Vandekerckhove

Bibliographic Information

  • Book Title: Gel-Free Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Kris Gevaert, Joël Vandekerckhove

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-148-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-147-5Published: 30 May 2011

  • Softcover ISBN: 978-1-4939-5819-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-148-2Published: 21 May 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 400

  • Topics: Protein Science, Proteomics

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