Plant Immunity

1. Front Matter

   Pages i-xv

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2. Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

   • Patrick Cournoyer, S. P. Dinesh-Kumar

   Pages 1-8

3. Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function

   • Peter Moffett

   Pages 9-20

4. Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

   • Yiping Qi, Fumiaki Katagiri

   Pages 21-30

5. Biochemical Purification of Native Immune Protein Complexes

   • James M. Elmore, Gitta Coaker

   Pages 31-44

6. Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity

   • Mario Roccaro, Imre E. Somssich

   Pages 45-58

7. Dose–Response to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in Arabidopsis

   • Xueqing Geng, David Mackey

   Pages 59-68

8. Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis

   • Xue-Cheng Zhang, Walter Gassmann

   Pages 69-77

9. Constructing Haustorium-Specific cDNA Libraries from Rust Fungi

   • Ann-Maree Catanzariti, Rohit Mago, Jeff Ellis, Peter Dodds

   Pages 79-87

10. Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes

    • Richard S. Hussey, Guozhong Huang, Rex Allen

    Pages 89-107

11. Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains

    • Brian H. Kvitko, Alan Collmer

    Pages 109-128

12. A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores

    • Edgar Huitema, Matthew Smoker, Sophien Kamoun

    Pages 129-135

13. Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis

    • John M. McDowell, Troy Hoff, Ryan G. Anderson, Daniel Deegan

    Pages 137-151

14. Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment

    • Shiv D. Kale, Brett M. Tyler

    Pages 153-172

15. Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast

    • Yuanchao Wang, Qian Huang

    Pages 173-180

16. Purification of Effector–Target Protein Complexes via Transient Expression in Nicotiana benthamiana

    • Joe Win, Sophien Kamoun, Alexandra M. E. Jones

    Pages 181-194

17. Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue

    • Petra C. Boevink, Paul R. J. Birch, Stephen C. Whisson

    Pages 195-209

18. Immunolocalization of Pathogen Effectors

    • Eric Kemen, Kurt Mendgen, Ralf T. Voegele

    Pages 211-225

19. Laser Capture Microdissection of Nematode Feeding Cells

    • Nagabhushana Ithal, Melissa G. Mitchum

    Pages 227-240

20. Laser Microdissection of Plant–Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling

    • Divya Chandran, Noriko Inada, Mary C. Wildermuth

    Pages 241-262

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes.  In  Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens.  These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.  Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

• Disease control
• Immune signaling
• Laser microdissection
• Molecular mechanisms
• Pathogen effector proteins
• Pathosystems
• Plant-microbe interactions

From the reviews:

“This volume … describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. … Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field.” (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)

• , Plant Pathology, Physiol., & Weed Sci., Virginia Tech, Blacksburg, USA

  John M. McDowell

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