Overview
- Editors:
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Ella Palmer
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MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, London, United Kingdom
- Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand upon them -Includes tips from the experts highlighting potential methodological pitfalls in order to ensure successful implementation
- Includes supplementary material: sn.pub/extras
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Table of contents (16 protocols)
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- Ella Palmer, Tom C. Freeman
Pages 27-40
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- Tanya M. Redmond, Michael D. Uhler
Pages 41-52
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- Holger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, Vytaute Starkuviene
Pages 73-81
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- Wei Niu, G. Traver Hart, Edward M. Marcotte
Pages 83-95
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- Hansjürgen Volkmer, Frank Weise
Pages 97-106
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- Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, Michael Stürzl
Pages 107-118
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- Baochuan Lin, James B. Delehanty
Pages 119-137
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- Yoann Roupioz, Sarah Milgram, André Roget, Thierry Livache
Pages 139-149
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- Satoshi Yamaguchi, Erika Matsunuma, Teruyuki Nagamune
Pages 151-157
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- Andrew L. Hook, Nicolas H. Voelcker, Helmut Thissen
Pages 159-170
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- Salvatore Pernagallo, Juan J. Diaz-Mochon
Pages 171-180
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- Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, Robert Damoiseaux
Pages 191-206
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Back Matter
Pages 207-209
About this book
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
Editors and Affiliations
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MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, London, United Kingdom
Ella Palmer
