Nitric Oxide

1. Front Matter

   Pages i-xi

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2. Nitric Oxide Physiology and Pathology

   • David G. Hirst, Tracy Robson

   Pages 1-13

3. Detection and Quantification of Nitric Oxide

   1. Front Matter

      Pages 15-15

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   2. Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide

      • Kai Rothkamm, Susanne Burdak-Rothkamm

      Pages 17-25

   3. Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescence

      • Enika Nagababu, Joseph M. Rifkind

      Pages 27-37

   4. Measurement of Nitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin Oxidation Assay

      • Barbora Piknova, Alan N. Schechter

      Pages 39-56

   5. Selective Fluorescent Activation for Bioimaging the Expression of Nitric Oxide in Cellular and In Vivo Systems

      • Junfeng Zhang, Hao Hong

      Pages 57-71

   6. Real-Time Measurement of Murine Hippocampus NO Levels in Response to Cerebral Ischemia/ Reperfusion

      • Xiaoxiang Zheng, Kezhou Liu, Yong Yang

      Pages 73-80

   7. Detection of Low Levels of Nitric Oxide Using an Electrochemical Sensor

      • Yong Chool Boo, Gyeong In Mun, Sarah L. Tressel, Hanjoong Jo

      Pages 81-89

   8. Determination of the Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies

      • Marcela A. Segundo, Luís M. Magalhães, Joana P.N. Ribeiro, Marlene Lúcio, Salette Reis

      Pages 91-104

   9. Aqueous Measurement of Nitric Oxide Using Membrane Inlet Mass Spectrometry

      • David N. Silverman, Chingkuang Tu

      Pages 105-114

   10. Quantum Cascade Laser Technology for the Ultrasensitive Detection of Low-Level Nitric Oxide

       • Angela Elia, Pietro Mario Lugarà, Cinzia Di Franco, Vincenzo Spagnolo

       Pages 115-133

   11. Determination of In Vivo Nitric Oxide Levels in Animal Tissues Using a Novel Spin Trapping Technology

       • Anatoly F. Vanin, Alexander A. Timoshin

       Pages 135-149

4. Nitric Oxide Generation

   1. Front Matter

      Pages 151-151

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   2. β Cell Protection by Inhibition of iNOS Through Lentiviral Vector-Based Strategies

      • Sean O. Hynes, Cillian McCabe, Timothy O’Brien

      Pages 153-168

   3. Characterization of Nitric Oxide Delivery Systems Produced By Various Nanotechnologies

      • Chi H. Lee

      Pages 169-185

   4. Nitric Oxide Releasing Nanoparticle Synthesis and Characterization

      • George Han, Adam J. Friedman, Joel M. Friedman

      Pages 187-195

   5. NOS Antagonism Using Viral Vectors as an Experimental Strategy: Implications for In Vivo Studies of Cardiovascular Control and Peripheral Neuropathies

      • Beihui Liu, James Hewinson, Haibo Xu, Francisco Montero, Carmen R. Sunico, Federico Portillo et al.

      Pages 197-223

5. Back Matter

   Pages 225-226

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The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.

• Biofilms
• Detection and quantification
• In vivo generation
• Nanoparticles
• Nitrate
• Physiological, pathological responses
• Viral, shRNA delivery systems

• School of Pharmacy, Queen's University, Belfast, Belfast, United Kingdom

  Helen O. McCarthy, Jonathan A. Coulter

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