Flow Cytometry Protocols

1. Front Matter

   Pages i-xii

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2. Flow Cytometry: An Introduction

   • Alice L. Givan

   Pages 1-29

3. Breaking the Dimensionality Barrier

   • C. Bruce Bagwell

   Pages 31-51

4. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

   • Lili Wang, Adolfas K. Gaigalas, Ming Yan

   Pages 53-65

5. Quantum Dots for Quantitative Flow Cytometry

   • Tione Buranda, Yang Wu, Larry A. Sklar

   Pages 67-84

6. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry

   • Henri C. van der Heyde, Irene Gramaglia

   Pages 85-95

7. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization

   • Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, Amitabh Gaur

   Pages 97-118

8. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry

   • Joseph D. Tario Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, Paul K. Wallace

   Pages 119-164

9. Multiparameter Intracellular Cytokine Staining

   • Patricia Lovelace, Holden T. Maecker

   Pages 165-178

10. Phospho Flow Cytometry Methods for the Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples

    • Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, Garry P. Nolan

    Pages 179-202

11. Multiparametric Analysis of Apoptosis by Flow Cytometry

    • William G. Telford, Akira Komoriya, Beverly Z. Packard, C. Bruce Bagwell

    Pages 203-227

12. Multiparameter Cell Cycle Analysis

    • James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan

    Pages 229-249

13. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue

    • Ludovic Zimmerlin, Vera S. Donnenberg, Albert D. Donnenberg

    Pages 251-273

14. Flow Cytometry-Based Identification of Immature Myeloerythroid Development

    • Cornelis J. H. Pronk, David Bryder

    Pages 275-293

15. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia

    • Katherine R. Calvo, Catharine S. McCoy, Maryalice Stetler-Stevenson

    Pages 295-316

16. Flow Cytometry Assays in Primary Immunodeficiency Diseases

    • Maurice R. G. O’Gorman, Joshua Zollett, Nicolas Bensen

    Pages 317-335

17. Flow Cytometric Analysis of Microparticles

    • Henri C. van der Heyde, Irene Gramaglia, Valéry Combes, Thaddeus C. George, Georges E. Grau

    Pages 337-354

18. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling

    • Rita L. Strack, Robert J. Keenan, Benjamin S. Glick

    Pages 355-370

19. Flow Cytometric FRET Analysis of Protein Interaction

    • György Vereb, Péter Nagy, János Szöllo˝si

    Pages 371-392

20. Fluorescent Protein-Assisted Purification for Gene Expression Profiling

    • M. Raza Zaidi, Chi-Ping Day, Glenn Merlino

    Pages 393-405

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

• Cell sorting
• Combinatorial antibody profiling
• Diagnostic medicine
• Fluorescent proteins
• Imaging
• Immunophenotyping
• Phospho-flow
• Polychromatic characterizations
• Quantum dots

• Medical Center, Flow Cytometry Core Facility, George Washington University, Washington, USA

  Teresa S. Hawley

• Medical Center, Dept. Anatomy & Regenerative Biology, George Washington University, Washington, USA

  Robert G. Hawley

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