Editors:
- Comprehensive phospho-proteomics laboratory manual and research guide for both novice and experienced researchers
- Provides step-by-step protocols written by leading researchers in the field that are fully adaptable to any specific phospho-proteome analysis task
- Includes two detailed critical reviews on qualitative and quantitative mass-spectrometry-based phospho-proteomic analysis
- Covers both basic and state-of-the-art practical procedures as well as bio-informatics approaches to explore the phospho-proteome
- A compendium of cutting-edge protocols for the identification of phospho-proteins
Part of the book series: Methods in Molecular Biology (MIMB, volume 527)
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Table of contents (23 protocols)
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Front Matter
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Protein Phosphorylation and 2D Gel Electrophoresis
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Enrichment of Phosphoproteins and Peptides
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Phosphoprotein Analysis by Mass Spectrometry
About this book
Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Protocols, expert researchers contribute both well-established protocols and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Protocols is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future.
Editors and Affiliations
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Division of Toxicology, Leiden/Amsterdam Center for Drug Research (LACDR), Leiden University, Leiden, Netherlands
Marjo de Graauw
Bibliographic Information
Book Title: Phospho-Proteomics
Book Subtitle: Methods and Protocols
Editors: Marjo de Graauw
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-834-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-833-1Published: 05 March 2009
Softcover ISBN: 978-1-61737-917-8Published: 19 November 2010
eBook ISBN: 978-1-60327-834-8Published: 25 February 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 332
Number of Illustrations: 69 b/w illustrations
Topics: Protein Science, Proteomics