Overview
- Describes novel probes for detecting ROS and RNS
- Provides theoretical models to predict structural features of polyphenol antioxidants
- Provides automated assays for ROS metabolites and antioxidant capacity
- Use of HPLC-nanoHPLC-ESR-MS-ESI-ECD and high-voltage capillary electrophoresis
- Shows how correlative transmission microscopy allows for in-situ visualization of localized sites of oxidative stress
- Illustrates immunohistochemistry and bioluminescence techniques
- Detecting extracellular ROS by microdialysis
- Describes microarray technology
- Production of antibodies to detergent-resistant lipid rafts
- Stress response and gene expression
- Discusses antioxidant modeling, liposome preparation , response element activity and preparation of multi-antioxidant pharmaceutical formulations
- Describes HDL-associated paraoxonase enzyme activity in knock-out and transgenic mice
Part of the book series: Methods in Molecular Biology (MIMB, volume 477)
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Table of contents (31 protocols)
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Front Matter
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Reactive Oxygen and Nitrogen Techniques
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Front Matter
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Antioxidant Technology and Application
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Front Matter
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Keywords
About this book
Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Protocols in Oxidative Stress, all part of the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and ensuring replication of technology.
Cutting-edge and convenient, Advanced Protocols in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.
Editors and Affiliations
Bibliographic Information
Book Title: Advanced Protocols in Oxidative Stress I
Editors: Donald Armstrong
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-517-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-60327-218-6Published: 09 October 2008
Softcover ISBN: 978-1-61737-881-2Published: 20 January 2011
eBook ISBN: 978-1-60327-517-0Published: 14 December 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 430
Number of Illustrations: 99 b/w illustrations, 1 illustrations in colour
Topics: Biochemistry, general, Cell Biology, Oxidative Stress