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  • © 2008

Plant Embryogenesis

  • Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips
  • Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism
  • Provides the most comprehensive collection of protocols on plan embryo development

Part of the book series: Methods in Molecular Biology (MIMB, volume 427)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
  2. Model Embryonic Systems

    1. Front Matter

      Pages 1-1
    2. Arabidopsis Embryogenesis

      • Soomin Park, John J. Harada
      Pages 3-16
    3. Maize Embryogenesis

      • Pilar Fontanet, Carlos M. Vicient
      Pages 17-29
    4. Spruce Embryogenesis

      • Sara von Arnold, David Clapham
      Pages 31-47
  3. Cellular, Genetic and Molecular Mechanisms of Plant Embryogenesis

    1. Front Matter

      Pages 49-49
    2. In Vitro Fertilization With Isolated Higher Plant Gametes

      • Erhard Kranz, Yoichiro Hoshino, Takashi Okamoto
      Pages 51-69
    3. In Vitro Culture of Arabidopsis Embryos

      • Michael Sauer, Jiří Friml
      Pages 71-76
    4. Culture and Time-Lapse Tracking of Barley Microspore-Derived Embryos

      • Simone de F. Maraschin, Sandra van Bergen, Marco Vennik, Mei Wang
      Pages 77-89
    5. Isolation of Embryo-Specific Mutants in Arabidopsis

      • Nai-You Liu, Zhi-Feng Zhang, Wei-Cai Yang
      Pages 91-100
    6. Isolation of Embryo-Specific Mutants in Arabidopsis

      • Nai-You Liu, Zhi-Feng Zhang, Wei-Cai Yang
      Pages 101-109
    7. Laser-Capture Microdissection to Study Global Transcriptional Changes During Plant Embryogenesis

      • Stuart A. Casson, Matthew W.B. Spencer, Keith Lindsey
      Pages 111-120
    8. Promoter Trapping System to Study Embryogenesis

      • Robert Blanvillain, Patrick Gallois
      Pages 121-135
    9. Visualization of Auxin Gradients in Embryogenesis

      • Michael Sauer, Jiří Friml
      Pages 137-144
    10. Intercellular Trafficking of Macromolecules During Embryogenesis

      • Insoon Kim, Patricia C. Zambryski
      Pages 145-155
    11. Immunolocalization of Proteins in Somatic Embryos

      • Andrei P. Smertenko, Patrick J. Hussey
      Pages 157-171
    12. Detection of Programmed Cell Death in Plant Embryos

      • Lada H. Filonova, María F. Suárez, Peter V. Bozhkov
      Pages 173-179
  4. Back Matter

    Pages 181-184

About this book

The advent of the postgenome era has brought a remarkable evolution in modern plant embryology. In Plant Embryogenesis, the authors focus on the common developmental process and compile novel tools and methods designed specifically for studying plant embryogenesis. In each chapter, detailed outlines of model embryonic systems are provided, as well as cutting-edge cellular, genetic and molecular mechanisms. This important book gives insight into the methods used in studying the transformation of phylogenetically-conserved embryonic patterns into flora’s astounding diversity of growth forms today. Invaluable to those new to the field and inspiring to full-fledged plant embryologists, Plant Embryogenesis provides today’s most comprehensive platform to the understanding of plant embryo development.

Reviews

From the reviews:

"I wish to recommend … ‘Plant Embryogenesis’ edited by M.F. Suárez and P.V. Bozhkov. … we can whole-heartedly express our thanks to all authors – leading experts in the field of plant embryology for very useful book. … The authors aim this book primarily at researchers who are relatively new to the field of plant embryology. … this volume will be very useful text in all laboratories dealing with plant development and embryology. It can inspire students as well as full-fledged plant embryologists." (Z. Vondráková, Biologia Plantarum, Vol. 52 (2), 2008)

Editors and Affiliations

  • Departamento de Biología Molecular y Bioquímica Facultad de Ciencias, Universidad de Málaga, Málaga, Spain

    María F. Suárez

  • Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Peter V. Bozhkov

Bibliographic Information

  • Book Title: Plant Embryogenesis

  • Editors: María F. Suárez, Peter V. Bozhkov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-273-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-931-4Published: 17 December 2007

  • Softcover ISBN: 978-1-61737-848-5Published: 19 November 2010

  • eBook ISBN: 978-1-59745-273-1Published: 07 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 184

  • Number of Illustrations: 21 b/w illustrations

  • Topics: Plant Genetics and Genomics, Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access