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Hematopoietic Stem Cell Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Kevin D. Bunting

    1. Department of Medicine, Division of Hematology & Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, USA

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  • Provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations
  • Offers a variety of transplantation approaches to measure HSC function in vivo
  • Details retroviral and lentiviral transduction procedures and analysis of integration sites
  • Describes assays using embryonic and adult tissues to derive HSC

Part of the book series: Methods in Molecular Biology (MIMB, volume 430)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Expanding Tool Kit for Hematopoietic Stem Cell Research

      • William Tse, Kevin D. Bunting
      Pages 3-18

  3. Stem Cell Enrichment and Analysis

    1. Front Matter

      Pages 19-19
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    2. Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties

      • Tarja A. Juopperi, Saul J. Sharkis
      Pages 21-30

    3. Mobilization of Hematopoietic Stem and Progenitor Cells in Mice

      • Simon N. Robinson, Ronald P. van Os
      Pages 31-53

    4. Transmigration of Human CD34+ Cells

      • Seiji Fukuda, Louis M. Pelus
      Pages 55-75

    5. Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells

      • Hongmei Shen, Matthew Boyer, Tao Cheng
      Pages 77-86

    6. Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry

      • William G. Kerr
      Pages 87-99

  4. In Vitro Assays and Differentiation

    1. Front Matter

      Pages 101-101
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    2. In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells

      • Jinhua Shen, Cheng-Kui Qu
      Pages 103-118

    3. Hematopoietic Development of Human Embryonic Stem Cells in Culture

      • Xinghui Tian, Dan S. Kaufman
      Pages 119-133

    4. In Vitro Human T Cell Development Directed by Notch–Ligand Interactions

      • Génève Awong, Ross N. La Motte-Mohs, Juan Carlos Zúñiga-Pflücker
      Pages 135-142

    5. In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C

      • Ronald P. van Os, Bertien Dethmers-Ausema, Gerald de Haan
      Pages 143-157

  5. Transplantation Assays for Mouse and Human HSC

    1. Front Matter

      Pages 159-159
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    2. Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection

      • Yuxia Zhan, Yi Zhao
      Pages 161-169

    3. Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects

      • Zhengqi Wang, Kevin D. Bunting
      Pages 171-181

    4. Isolation and Functional Characterization of Side Population Stem Cells

      • Jonathan B. Johnnidis, Fernando D. Camargo
      Pages 183-193

    5. Transplantation of Chimeric Fetal Liver to Study Hematopoiesis

      • Sigrid Eckardt, K. John McLaughlin
      Pages 195-211

    6. Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair

      • Ping Zhou, Sarah Hohm, Ben Capoccia, Louisa Wirthlin, David Hess, Dan Link et al.
      Pages 213-225
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Keywords

About this book

The hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways. Major advances in the isolation of HSC and progenitor subsets have been sparked by the gene expression array technologies, leading to the identification of new markers for prospective isolation. The first edition of Hematopoietic Stem Cell Protocols is a very thorough resource covering historically the basic techniques that have been published extensively over many years. To put together, a follow-up to this excellent book has been a hard act to follow. The intent of this book is to be distinct and not duplicate but to build on the strong basic assays and include updated approaches for the combination of phenotypic and functional analyses. This second edition of Hematopoietic Stem Cell Protocols thus aims to provide timely protocols developed in the HSC field since the last comprehensive review in this series. The approaches described in this book should be readily useful for the basic science researcher, especially those familiar with the use of mouse genetic models. The chapters in this book are geared toward the laboratory-based researcher and the development of pre-clinical studies to apply HSCs toward treating blood diseases more effectively.

Editors and Affiliations

  • Department of Medicine, Division of Hematology & Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, USA

    Kevin D. Bunting

Bibliographic Information

  • Book Title: Hematopoietic Stem Cell Protocols

  • Editors: Kevin D. Bunting

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-182-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-868-3Published: 31 January 2008

  • Softcover ISBN: 978-1-61737-816-4Published: 16 December 2010

  • eBook ISBN: 978-1-59745-182-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 312

  • Topics: Biochemistry, general, Cell Biology, Hematology, Molecular Medicine

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