Skip to main content
SpringerLink
Log in
Book cover

Exocytosis and Endocytosis

  • Book
  • © 2008

Overview

Editors:
  1. Andrei I. Ivanov

    1. Department of Medicine Gastroenterology and Hepatology Division, University of Rochester School of Medicine and Dentistry, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides classic, state of art, and cutting edge methods in a simple, easily reproducible format
  • Contains a comprehensive set of experimental models including cell-free systems, different specialized cells, and model organisms
  • Presents detailed lists of tested reagents that really work
  • Notes on troubleshooting from leading laboratories in the field

Part of the book series: Methods in Molecular Biology (MIMB, volume 440)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. An Overview of Methods for Analyzing Vesicular Trafficking

    1. Vesicular Trafficking: Molecular Tools and Targets

      • Elena V. Vassilieva, Asma Nusrat
      Pages 3-14

    2. Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful?

      • Andrei I. Ivanov
      Pages 15-33

  3. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis

    1. In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion

      • Susanne Kreye, Jörg Malsam, Thomas H. Söllner
      Pages 37-50

    2. Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells

      • Thorsten Lang
      Pages 51-59

    3. FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg

      • Julian L. Wong, Gary M. Wessel
      Pages 61-76

    4. A Cell-Free Assay for Endocytosis of E-Cadherin

      • Toshiaki Sakisaka, Yoshimi Takai
      Pages 77-87

    5. Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin

      • Noriyuki Nishimura, Takuya Sasaki
      Pages 89-96

    6. Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation

      • Xuhang Li, Mark Donowitz
      Pages 97-110

    7. Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses

      • Mark Kail, Angelika Barnekow
      Pages 111-125

  4. Analysis of Endocytosis and Exocytosis in Different Cell Systems

    1. High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins

      • Roland Govers, David E. James, Adelle C.F. Coster
      Pages 129-146

    2. Studying Phagocytosis by Live-Cell Scintillation Proximity Assay

      • Walter Stockinger, Axel Nohturfft
      Pages 147-155

    3. Transcytosis of Polymeric Immunoglobulin A in Polarized Madin–Darby Canine Kidney Cells

      • Asli Oztan, Christine Rondanino, Gerard Apodaca
      Pages 157-170

    4. The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells

      • Martin B.A. ter Beest
      Pages 171-186

    5. Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins

      • Véronique Proux-Gillardeaux, Thierry Galli
      Pages 187-201

    6. Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor

      • Munekazu Yamakuchi, Marcella Ferlito, Craig N. Morrell, Kenji Matsushita, Craig A. Fletcher, Wangsen Cao et al.
      Pages 203-215

    7. Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization

      • Xia Ding, Fang Wu, Zhen Guo, Xuebiao Yao
      Pages 217-226

    8. Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay

      • Holger Lorenz, Dale W. Hailey, Jennifer Lippincott-Schwartz
      Pages 227-233

    9. Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy

      • Alan R. Burns, Janet M. Oliver, Janet R. Pfeiffer, Bridget S. Wilson
      Pages 235-245

    10. Analyzing Endosomes in Nonsectioned Cells by Transmission Electron Microscopy

      • Willem Stoorvogel
      Pages 247-257
Back to top

Keywords

About this book

Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date,step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biology™ series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls.

Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.

Editors and Affiliations

  • Department of Medicine Gastroenterology and Hepatology Division, University of Rochester School of Medicine and Dentistry, Rochester, USA

    Andrei I. Ivanov

Bibliographic Information

  • Book Title: Exocytosis and Endocytosis

  • Editors: Andrei I. Ivanov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-178-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-865-2Published: 17 December 2007

  • Softcover ISBN: 978-1-61737-813-3Published: 10 December 2010

  • eBook ISBN: 978-1-59745-178-9Published: 15 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 412

  • Topics: Cell Biology, Human Physiology, Developmental Biology, Immunology, Neurobiology

Publish with us

Policies and ethics

Back to top

Search

Navigation