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  • © 2008

Autophagosome and Phagosome

Editors:

  • Autophagosome and phagosome methods for novices and advanced researchers alike
  • Vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases
  • Contains molecular, genetic, biochemical, imaging, cellular, animal, and ultrastructural analyses
  • Autophagy essentials for researchers working on apoptosis and cell death who want to add autophagy to their studies

Part of the book series: Methods in Molecular Biology (MIMB, volume 445)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvii
  2. Autophagosome and Phagosome

    • Vojo Deretic
    Pages 1-10
  3. Fine Structure of the Autophagosome

    • Eeva-Liisa Eskelinen
    Pages 11-28
  4. Methods for Assessing Autophagy and Autophagic Cell Death

    • Ezgi Tasdemir, Lorenzo Galluzzi, M. Chiara Maiuri, Alfredo Criollo, Ilio Vitale, Emilie Hangen et al.
    Pages 29-76
  5. LC3 and Autophagy

    • Isei Tanida, Takashi Ueno, Eiki Kominami
    Pages 77-88
  6. Amino Acid Regulation of Autophagosome Formation

    • Alfred J. Meijer
    Pages 89-109
  7. Autophagic Proteolysis of Long-Lived Proteins in Nonliver Cells

    • Esteban A. Roberts, Vojo Deretic
    Pages 111-117
  8. Autophagosomes in GFP-LC3 Transgenic Mice

    • Noboru Mizushima, Akiko Kuma
    Pages 119-124
  9. Experimental Control and Characterization of Autophagy in Drosophila

    • Gabor Juhasz, Thomas P. Neufeld
    Pages 125-133
  10. Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy

    • Julie E. Legakis, Daniel J. Klionsky
    Pages 135-145
  11. Protein Trafficking into Autophagosomes

    • Andrew Young, Sharon Tooze
    Pages 147-157
  12. Sphingolipids in Macroautophagy

    • Grégory Lavieu, Francesca Scarlatti, Giusy Sala, Stéphane Carpentier, Thierry Levade, Riccardo Ghidoni et al.
    Pages 159-173
  13. Molecular Links Between Autophagy and Apoptosis

    • Iwona A. Ciechomska, Christoph G. Goemans, Aviva M. Tolkovsky
    Pages 175-193
  14. Clearance of Mutant Aggregate-Prone Proteins by Autophagy

    • Brinda Ravikumar, Sovan Sarkar, David C. Rubinsztein
    Pages 195-211
  15. Chaperone-Mediated Autophagy

    • S. Kaushik, A. M. Cuervo
    Pages 227-244
  16. Microautophagy in the Yeast Saccharomyces cerevisiae

    • Andreas Uttenweiler, Andreas Mayer
    Pages 245-259
  17. EM Analysis of Phagosomes

    • Chantal de Chastellier
    Pages 261-285
  18. In Vitro Phagosome–Endosome Fusion

    • Isabelle Vergne, Vojo Deretic
    Pages 301-309

About this book

Autophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which could benefit greatly from updated methods, in order to prompt further explorations of their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two.

Reviews

From the reviews:

"The book is well balanced between autophagy and phagosomes, with an approximately equal number of chapters dedicated to each topic. … the book is a useful and comprehensive resource for people interested in the study of autophagy and phagocytosis. … scientists involved in apoptosis research may also find this book useful." (Franco H. Falcone, Immunology News, November, 2008)

Editors and Affiliations

  • Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, USA

    Vojo Deretic

Bibliographic Information

  • Book Title: Autophagosome and Phagosome

  • Editors: Vojo Deretic

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-157-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-853-9Published: 24 April 2008

  • Softcover ISBN: 978-1-61737-806-5Published: 09 December 2010

  • eBook ISBN: 978-1-59745-157-4Published: 19 April 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 455

  • Number of Illustrations: 96 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology, Molecular Medicine, Microbiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access