Drosophila

1. Front Matter

   Pages i-xi

   PDF

2. Drosophila melanogaster and the Development of Biology in the 20th Century

   • Alfonso Martinez Arias

   Pages 1-25

3. Getting Started

   • Hugo Stocker, Peter Gallant

   Pages 27-44

4. FlyBase

   • Rachel Drysdale, the FlyBase Consortium

   Pages 45-59

5. The Use of P-Element Transposons to Generate Transgenic Flies

   • André Bachmann, Elisabeth Knust

   Pages 61-77

6. The GAL4 System

   • David A. Elliott, Andrea H. Brand

   Pages 79-95

7. P-Element Mutagenesis

   • Thomas Hummel, Christian Klämbt

   Pages 97-117

8. EMS Screens

   • Christian Bökel

   Pages 119-138

9. RNAi Screening in Cultured Drosophila Cells

   • Sandra Steinbrink, Michael Boutros

   Pages 139-153

10. Methods for Homologous Recombination in Drosophila

    • Keith A. Maggert, Wei J. Gong, Kent G. Golic

    Pages 155-174

11. Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis

    • Johannes Bischof, Konrad Basler

    Pages 175-195

12. Cuticle Preparation of Drosophila Embryos and Larvae

    • Cyrille Alexandre

    Pages 197-205

13. Immunolabeling of Embryos

    • H.-Arno J. Müller

    Pages 207-218

14. Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins

    • Matthieu Cavey, Thomas Lecuit

    Pages 219-238

15. Analysis of Neuromuscular Junctions

    • Andreas Schmid, Stephan J. Sigrist

    Pages 239-251

16. Immunolabeling of Imaginal Discs

    • Thomas Klein

    Pages 253-263

17. Imaging Drosophila Pupal Wing Morphogenesis

    • Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, Suzanne Eaton

    Pages 265-275

18. Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections

    • Konstantin Gaengel, Marek Mlodzik

    Pages 277-287

19. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues

    • Eric Lécuyer, Neela Parthasarathy, Henry M. Krause

    Pages 289-302

20. Linear RNA Amplification for the Production of Microarray Hybridization Probes

    • Ansgar Klebes, Thomas B. Kornberg

    Pages 303-317

Drosophila melanogaster has been the model system of choice for many inv- tigators over the past hundred years. Due to its long-standing tradition as a model organism, many techniques used in Drosophila have been established and c- tinue to be developed to answer a variety of scientific questions. The recent inv- tion of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination are two important demonst- tions of the ongoing efforts to broaden the technical repertoire at hand for researchers working with Drosophila. This volume is a collection of protocols covering both standard techniques as well as novel methods. An introductory chapter highlights the importance of Drosophila as a model system for the dev- opment of Biology in the 20th century and review chapters provide concise and up-to-date overviews on selected experimental systems. This book makes no attempt to be comprehensive. A number of frequently used standard techniques were selected and are described in-depth to allow novices to get started with Drosophila. In addition, detailed protocols of recently developed methods that we felt will become of broad use in the Drosophila community within the next years are included. I am grateful to Konrad Basler, Suzanne Eaton, Elisabeth Knust, Pavel Tomancak, and Andreas Wodarz for alerting me to novel techniques and for their advice on selecting the protocols for this book. I would also like to thank all the authors for their expert contribution to this Volume. Christian Dahmann v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. .

• Homologous recombination
• Microarray
• Model organisms
• cell lines
• genes
• hybridization
• molecular biology
• Entomology

• MPI für Molekulare Zellbiologie und Genetik, Dresden, Germany

  Christian Dahmann

Policies and ethics