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High Content Screening

  • Book
  • © 2006

Overview

Editors:
  1. D. Lansing Taylor

    1. Cellumen, Inc., Pittsburgh

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Jeffrey R. Haskins

    1. Cellomics, Inc., Pittsburgh

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Kenneth A. Giuliano

    1. Cellumen, Inc., Pittsburgh

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 356)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiii
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  3. Instrumentation, Biological Application Software, and Sample Preparation

    1. Front Matter

      Pages 39-39
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    2. Requirements, Features, and Performance of High Content Screening Platforms

      • Albert H. Gough, Paul A. Johnston
      Pages 41-61

    3. Characteristics and Value of Directed Algorithms in High Content Screening

      • Richik N. Ghosh, Oleg Lapets, Jeffrey R. Haskins
      Pages 63-81

    4. Characteristics and Value of Machine Learning for Imaging in High Content Screening

      • Juergen A. Klenk
      Pages 83-94

    5. Tools for Quantitative and Validated Measurements of Cells

      • Anne L. Plant, John T. Elliott, Alessandro Tona, Dennis McDaniel, Kurt J. Langenbach
      Pages 95-107

    6. Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays

      • Gillian R. Richards, Julie E. Kerby, Grace K. Y. Chan, Peter B. Simpson
      Pages 109-119

    7. Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications

      • Daniel R. Marshak, Dale E. Greenwalt
      Pages 121-128

    8. Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel

      • Oren Beske, Daniel Bassoni, Simon Goldbard
      Pages 129-138

  4. Reagents

    1. Front Matter

      Pages 139-139
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    2. Reagents to Measure and Manipulate Cell Functions

      • Kenneth A. Giuliano, D. Lansing Taylor, Alan S. Waggoner
      Pages 141-163

    3. Fluorescent Proteins and Engineered Cell Lines

      • Nick Thomas
      Pages 165-187

    4. Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays

      • Kenneth A. Giuliano
      Pages 189-193

    5. The HaloTag™

      • Georgyi V. Los, Keith Wood
      Pages 195-208

    6. Protein Labeling With FlAsH and ReAsH

      • Thomas Machleidt, Matt Robers, George T. Hanson
      Pages 209-220

    7. Exploiting Network Biology to Improve Drug Discovery

      • Marnie L. MacDonald, John K. Westwick
      Pages 221-232
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Keywords

About this book

There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has also demonstrated the need for both academic researchers and industrial researchers to now understand the functions of genes and gene products. The cell is the basic unit of life and it has been at the cellular level where function can be demonstrated most cost-effectively and rapidly. High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell.

Reviews

"...very useful."  -Weighted Numberical Score: 97 - 5  Stars!-Doody's Health Science Book Review Journal

Editors and Affiliations

  • Cellumen, Inc., Pittsburgh

    D. Lansing Taylor, Kenneth A. Giuliano

  • Cellomics, Inc., Pittsburgh

    Jeffrey R. Haskins

Bibliographic Information

  • Book Title: High Content Screening

  • Editors: D. Lansing Taylor, Jeffrey R. Haskins, Kenneth A. Giuliano

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597452173

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-731-0Published: 15 August 2006

  • Softcover ISBN: 978-1-61737-746-4Published: 10 December 2010

  • eBook ISBN: 978-1-59745-217-5Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 464

  • Number of Illustrations: 168 b/w illustrations

  • Topics: Cell Biology, Medicinal Chemistry

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