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  • Book
  • © 2007

Hedgehog Signaling Protocols

  • Offers various detailed chapters on experimental description, including anecdotal pointers and notes
  • Intended for Molecular Biologists, Biochemists, and Geneticists

Part of the book series: Methods in Molecular Biology (MIMB, volume 397)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xvii
  2. Purifying the Hedgehog Protein and its Variants

    • Darren P. Baker, Frederick R. Taylor, R. Blake Pepinsky
    Pages 1-22
  3. Application of Sonic Hedgehog to the Developing Chick Limb

    • Eva Tiecke, Cheryll Tickle
    Pages 23-33
  4. Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors

    • Thomas Hollemann, Emmanuel Tadjuidje, Katja Koebernick, Tomas Pieler
    Pages 35-45
  5. Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems

    • Maria Kasper, Gerhard Regl, Thomas Eichberger, Anna-Maria Frischauf, Fritz Aberger
    Pages 67-78
  6. Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues

    • Christine M. Bankers, Joan E. Hooper
    Pages 145-160
  7. Biochemical Fractionation of Drosophila Cells

    • Melanie Stegman, David Robbins
    Pages 203-213
  8. Back Matter

    Pages 251-259

About this book

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates. 

Editors and Affiliations

  • Deptment of Biomedical Sciences College of Medicine, Florida State University, Tallahassee, USA

    Jamila I. Horabin

Bibliographic Information

  • Book Title: Hedgehog Signaling Protocols

  • Editors: Jamila I. Horabin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-516-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-692-4Published: 13 September 2007

  • Softcover ISBN: 978-1-61737-721-1Published: 19 November 2010

  • eBook ISBN: 978-1-59745-516-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 256

  • Number of Illustrations: 38 b/w illustrations, 7 illustrations in colour

  • Topics: Cell Biology, Animal Genetics and Genomics, Human Genetics, Animal Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access