Overview
- Cutting edge methods that employ monoclonal antibodies in a clinical setting
- In-depth description for generating mouse and recombinant humanized antibodies
- Comprehensive review of how antibodies are being used in bead-based methods for measuring proteins
- Reviews of both flow cytometry and immunohistochemistry methods in diagnosis and classification of hematologic diseases
Part of the book series: Methods in Molecular Biology (MIMB, volume 378)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.
Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.
Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.
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Editors and Affiliations
Bibliographic Information
Book Title: Monoclonal Antibodies
Book Subtitle: Methods and Protocols
Editors: Maher Albitar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-323-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-567-5Published: 25 June 2007
Softcover ISBN: 978-1-61737-646-7Published: 19 November 2010
eBook ISBN: 978-1-59745-323-3Published: 02 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 228
Number of Illustrations: 71 b/w illustrations, 4 illustrations in colour
Topics: Immunology, Cancer Research, Molecular Medicine