Overview
- A selected description of techniques in neuropeptide research written by leaders in the field
- Design synthesis and biological evaluation of peptides and peptide analogues
- Emphasis to advanced technologies of transfection transgenic delivery and bioinformatics
- Primary cells and cell lines techniques in neuropeptide processing trafficking and secretion
- In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice
- Developmental milestones of behavior in newborn mice
Part of the book series: Neuromethods (NM, volume 39)
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Table of contents (10 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.
Reviews
From the reviews:
"Novel molecular techniques have been developed to better understand peptide function in health and disease. This book deals with the most recent protocols for investigation of peptide function. … Neuroscientists are the main audience. … With well written text and excellent diagrams, the book delves into the intricacies of peptide research, including tissue preparation, methods of DNA inoculation, and experimental mouse models of peptide research. … is a good book for neuroscience libraries." (Celso Agner, Doody’s Review Service, June, 2008)
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Neuropeptide Techniques
Editors: Illana Gozes
Series Title: Neuromethods
DOI: https://doi.org/10.1007/978-1-60327-099-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-468-5Published: 13 November 2007
Softcover ISBN: 978-1-61737-587-3Published: 09 December 2010
eBook ISBN: 978-1-60327-099-1Published: 06 February 2008
Series ISSN: 0893-2336
Series E-ISSN: 1940-6045
Edition Number: 1
Number of Pages: XII, 160
Number of Illustrations: 51 b/w illustrations, 1 illustrations in colour
Topics: Neurosciences, Proteomics, Protein Science, Human Physiology, Neurobiology