Editors:
- Offers a comprehensive, up-to-date collection of all the main strategies of experimental genome-scale gene essentiality analysis
- Contains a detailed description of the procedures as well as a discussion of potential difficulties and failures
- Includes chapters on statistical analysis of the generated datasets and their integration into existing genomic databases
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 416)
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Table of contents (31 protocols)
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Front Matter
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Introduction
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Front Matter
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Experimental Protocols
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Front Matter
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Populational Genome-Wide Essentiality Screens
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Systematic Collections of Knockout Mutants
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About this book
Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
Editors and Affiliations
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Burnham Institute for Medical Research, La Jolla
Andrei L. Osterman
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Fellowship for Interpretation of Genomes (FIG), Burr Ridge
Svetlana Y. Gerdes
Bibliographic Information
Book Title: Microbial Gene Essentiality: Protocols and Bioinformatics
Editors: Andrei L. Osterman, Svetlana Y. Gerdes
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-321-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-378-7Published: 04 February 2008
Softcover ISBN: 978-1-61737-529-3Published: 19 November 2010
eBook ISBN: 978-1-59745-321-9Published: 08 April 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 486
Topics: Biochemistry, general, Cell Biology, Human Genetics