Chemosensitivity

1. Front Matter

   Pages i-xiii

   PDF

2. An Overview of Chemosensitivity Testing

   • Rosalyn D. Blumenthal

   Pages 3-18

3. Clonogenic Cell Survival Assay

   • Anupama Munshi, Marvette Hobbs, Raymond E. Meyn

   Pages 21-28

4. High-Sensitivity Cytotoxicity Assays for Nonadherent Cells

   • M. Jules Mattes

   Pages 29-37

5. Sulforhodamine B Assay and Chemosensitivity

   • Wieland Voigt

   Pages 39-48

6. Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity

   • Gertjan J. L. Kaspers

   Pages 49-57

7. Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity

   • Hisayuki Kobayashi

   Pages 59-67

8. The MTT Assay to Evaluate Chemosensitivity

   • Jack D. Burton

   Pages 69-78

9. Histoculture Drug Response Assay to Monitor Chemoresponse

   • Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, Shiro Nozawa

   Pages 79-86

10. In Vitro Testing of Chemosensitivity in Physiological Hypoxia

    • Rita Grigoryan, Nino Keshelava, Clarke Anderson, C. Patrick Reynolds

    Pages 87-100

11. Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements

    • Christian M. Kurbacher, Ian A. Cree

    Pages 101-120

12. High-Throughput Technology

    • Marylène Fortin, Ann-Muriel Steff, Patrice Hugo

    Pages 121-137

13. Dimscan

    • Nino Keshelava, Tomás Frgala, Jirí Krejsa, Ondrej Kalous, C. Patrick Reynolds

    Pages 139-153

14. The ChemoFx® Assay

    • Robert L. Ochs, Dennis Burholt, Paul Kornblith

    Pages 155-172

15. Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models

    • C. Patrick Reynolds, Barry J. Maurer

    Pages 173-183

16. Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity

    • John F.Gibbs, Youcef M.Rustum, Harry K.Slocum

    Pages 185-195

17. Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing

    • Uriel Bachrach

    Pages 197-211

18. Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity

    • Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, Seiji Kawasaki

    Pages 213-227

19. Back Matter

    Pages 229-231

    PDF

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.

• cell
• cell culture
• cell death
• chemotherapy
• imaging
• tumor

• Garden State Cancer Center, Belleville

  Rosalyn D. Blumenthal

Policies and ethics