Overview
- Editors:
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Robert C. Dickson
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Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington
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Michael D. Mendenhall
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Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington
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Table of contents (21 protocols)
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- Elaine A. Elion, Yunmei Wang
Pages 1-14
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- Maofu Fu, Chenguang Wang, Xueping Zhang, Richard G. Pestell
Pages 15-36
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- Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, Natalie G. Ahn
Pages 37-49
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- Daniel J. Noonan, Kenneth Henry, Michelle L. Twaroski
Pages 51-65
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- Jan Brábek, Steven K. Hanks
Pages 79-90
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- Francesca Zappacosta, Michael J. Huddleston, Roland S. Annan
Pages 91-110
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- Paul C. Brandt, Thomas C. Vanaman
Pages 111-127
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- Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, Emery H. Bresnick
Pages 129-146
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- Natasha Elizabeth Zachara, Win Den Cheung, Gerald Warren Hart
Pages 175-194
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- Jaeho Lee, Donghang Cheng, Mark T. Bedford
Pages 195-208
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- Gil-Soo Han, George M. Carman
Pages 209-216
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- Gregory S. Taylor, Jack E. Dixon
Pages 217-227
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- Christina C. Leslie, Michael H. Gelb
Pages 229-242
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- Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, Daryll B. DeWald
Pages 243-258
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- Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, Manuela Zaccolo
Pages 259-270
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- Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, Albrecht G. von Arnim
Pages 271-286
About this book
In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.
Reviews
Reviews of the first edition:
...this well-organized volume is clearly among the most viable protocol books published in this field and will turn out to be a helpful tool for many laboratories.-FEBS Letters
