Editors:

David M. Terrian⁰

David M. Terrian
1. Brody School of Medicine, East Carolina University, Greenville
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Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 218)

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Table of contents (22 protocols)

Front Matter

Pages i-xiii

PDF
Manipulation and Detection of Survival Signals
1. Front Matter
  
  Pages 1-1
  
  PDF
2. Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
  
  Erik S. Knudsen, Steven P. Angus
  
  Pages 3-15
3. Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination
  
  Simon W. Hayward, Yuzhuo Wang, Mark L. Day
  
  Pages 17-33
4. Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells
  
  Hideki Kawasome, Takashi Hamazaki, Tetsuo Minamino, Naohiro Terada
  
  Pages 35-46
5. The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
  
  Richard A. Franklin
  
  Pages 47-57
6. Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
  
  Christopher W. Gregory
  
  Pages 59-69
7. Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
  
  Raymond R. Mattingly
  
  Pages 71-83
8. TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)
  
  Stephanie M. Oberhaus
  
  Pages 85-96
Manipulation and Edtection of Oncogenic Signals
1. Front Matter
  
  Pages 97-97
  
  PDF
2. Kinetworks™ Protein Kinase Multiblot Analysis
  
  Steven Pelech, Catherine Sutter, Hong Zhang
  
  Pages 99-111
3. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
  
  Wen-Chang Lin
  
  Pages 113-125
4. Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay
  
  Yan-Hua Chen, Qun Lu
  
  Pages 127-132
5. Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay
  
  Irwin H. Gelman
  
  Pages 133-141
6. Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells
  
  Daqing Wu, Ginger G. Wescott, David M. Terrian
  
  Pages 143-153
7. Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)
  
  Julian Gomez-Cambronero, Joel Horwitz, Ramadan I. Sha’afi
  
  Pages 155-176
8. Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model
  
  Yunfeng Zhao, Daret K. St. Clair
  
  Pages 177-184
9. Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer
  
  Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
  
  Pages 185-201
10. Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
  
  Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
  
  Pages 203-220
11. Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
  
  Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
  
  Pages 221-252

About this book

Cells respond to environmental cues through a complex and dynamic network of signaling pathways that normally maintain a critical balance between cellular proliferation, differentiation, senescence, and death. One current research challenge is to identify those aberrations in signal transd- tion that directly contribute to a loss of this division-limited equilibrium and the progression to malignant transformation. The study of cell-signaling m- ecules in this context is a central component of cancer research. From the knowledge of such targets, investigators have been able to productively advance many insightful hypotheses about how a particular cancer cell may misinterpret, or respond inappropriately to, growth regulatory cues in their environment. Despite these key insights, the rapidly evolving nature of cell signaling research in cancer has necessitated a continuous revision of these theoretical constructs and the updating of methods used in their study. One contemporary example of the evolution of this field is provided by an analysis of the Human Genome Project data, which reveal a previously unsuspected diversity in the multigene families encoding for most signaling pathway int- mediates. In assessing the usefulness of a particular methodological approach, therefore, we will need to keep in mind that there is a premium on those p- tocols that can be easily adapted for the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together several such methods in cell signaling research that are scientifically grounded within the cancer biology field.

Reviews

"...this volume is a very useful source for signal transduction protocols...This text should offer readers an excellent starting point for many important techniques in cancer signal transduction. Its ease of use and reasonable cost only add to its attraction." - Neuro-Oncology

Editors and Affiliations

Brody School of Medicine, East Carolina University, Greenville

David M. Terrian

Bibliographic Information

Book Title: Cancer Cell Signaling
Book Subtitle: Methods and Protocols
Editors: David M. Terrian
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592593569
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Hardcover ISBN: 978-1-58829-075-5Published: 09 December 2002
Softcover ISBN: 978-1-61737-341-1Published: 10 November 2010
eBook ISBN: 978-1-59259-356-9Published: 05 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 333
Topics: Oncology

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Cancer Cell Signaling

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Table of contents (22 protocols)

Front Matter

Manipulation and Detection of Survival Signals

Front Matter

Functional Analysis of the Antimitogenic Activity of Tumor Suppressors

Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination

Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells

The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress

Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression

Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells

TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)

Manipulation and Edtection of Oncogenic Signals

Front Matter

Kinetworks™ Protein Kinase Multiblot Analysis

Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers

Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay

Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay

Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells

Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)

Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model

Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer

Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes

Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes

About this book

Reviews

Editors and Affiliations

Brody School of Medicine, East Carolina University, Greenville

Bibliographic Information

Publish with us

Buy it now

Other ways to access

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Editors:

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Table of contents (22 protocols)

Front Matter

Manipulation and Detection of Survival Signals

Front Matter

Manipulation and Edtection of Oncogenic Signals

Front Matter

About this book

Reviews

Editors and Affiliations

Brody School of Medicine, East Carolina University, Greenville

Bibliographic Information

Publish with us

Buy it now

Buying options

Other ways to access

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