Apoptosis Techniques and Protocols

1. Front Matter

   Pages i-xv

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2. Molecular Characterization of the Proapoptotic Protein Bax

   • Yi-Te Hsu, Soraya Smaili

   Pages 1-20

3. Assessing Cytochrome-c Release from Mitochondria

   • Douglas W. Ethell, Douglas R. Green

   Pages 21-34

4. Monitoring Caspases in Neuronal Cell Death

   • Younes Bounhar, Omar Tounekti, Andréa C. LeBlanc

   Pages 35-59

5. HSV Amplicon Vectors in Neuronal Apoptosis Studies

   • Kathleen A. Maguire-Zeiss, William J. Bowers, Howard J. Federoff

   Pages 61-82

6. Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons

   • Yan Zhang, Andréa C. LeBlanc

   Pages 83-106

7. Designing Microarray Experiments for Neurobiology

   • P. Scott Eastman, Jeanne F. Loring

   Pages 107-132

8. Analysis of Gene Expression by Nylon Membrane cDNA Arrays

   • H. Michael Tucker, Steven Estus

   Pages 133-147

9. Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs

   • Mark D. Berry, Paula C. Ashe

   Pages 149-161

10. Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons

    • Barbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund et al.

    Pages 163-175

11. Detection and Analysis of Synaptosis

    • Greg M. Cole, Karen Gylys

    Pages 177-203

12. In Situ Detection of Apoptotic Neurons

    • Kevin A. Roth

    Pages 205-224

13. Apoptotic and Oxidative Indicators in Alzheimer’s Disease

    • Arun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu et al.

    Pages 225-246

14. Back Matter

    Pages 247-250

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The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature.
Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.

Review of the first edition:
"The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and protocols among all the diverse offerings of the book." Three Stars. -Doody's Health Sciences Book Review Journal

"...the book has updated many of the state-of-the-art techniques for analysis of neuronal apoptosis. More importantly, the book also offers the first-hand and step-by-step experimental details for molecular, cellular, biochemical and immunohistochemical assays of neuronal apoptotic death." - Biotechnic & Histochemistry

• Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada

  Andréa C. LeBlanc

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