Overview
- Editors:
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Donald Armstrong
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University of Florida, Gainesville
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Table of contents (36 protocols)
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Techniques for Histological Identification
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- E. Ann Ellis, Maria B. Grant
Pages 3-12
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- E. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, Donald Armstrong
Pages 13-17
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- Kiyoshi Akeo, Tadahisa Hiramitsu, Keiichi Watanabe
Pages 19-29
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- Hideyuki J. Majima, Takako Nakanishi-Ueda, Toshihiko Ozawa
Pages 31-34
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- Jürgen Frank, Alfonso Pompella, Hans K. Biesalski
Pages 35-40
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- Alfonso Pompella, Silvia Dominici, Jürgen Frank, Hans K. Biesalski
Pages 41-46
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Techniques for Subcellular Localization
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- R. Preston Mason, Robert F. Jacob
Pages 69-78
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- Kaori Kobayashi, Hiroshi Kobayashi
Pages 79-84
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- Tracey A. Ignatowski, Robert N. Spengler
Pages 85-96
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Techniques for Molecular Biology
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- Ahmad Aljada, Husam Ghanim, Paresh Dandona
Pages 99-103
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- Ahmad Aljada, Husam Ghanim, Paresh Dandona
Pages 105-110
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- Yoshikazu Ito, David A. Lipschitz
Pages 111-116
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- Gieri Camenisch, Roland H. Wenger, Max Gassmann
Pages 117-129
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About this book
In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with “oxidative stress” using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.
Reviews
"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS
Editors and Affiliations
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University of Florida, Gainesville
Donald Armstrong
