Overview
- Editors:
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Jack H. Mydlo
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Temple University School of Medicine, Philadelphia
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Table of contents (26 protocols)
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In Vitro Experimental Techniques
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- William W. Zhang, Laurence H. Klotz
Pages 55-67
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- Raffaele Baffa, Coleen M. Calviello, Teresa Druck, Leonard G. Gomella
Pages 81-93
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- Kenneth J. Pienta, Jeffrey F. Williams
Pages 95-100
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- Alexander Kugler, Paul Thelen, Rolf-Hermann Ringert
Pages 139-150
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- Greg L. Griewe, Robert Dean, Wei Zhang, Isabella A. Sesterhenn, Shiv Srivastava, Judd W. Moul
Pages 165-173
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- Leslie Kushner, Pui Yan Chiu, Peter Pinto, Gary Hal Weis
Pages 175-191
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- Allen Chernoff, Viera Kasparcova, W. Marston Linehan, Catherine A. Stolle
Pages 193-216
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- Robert L. Vessella, D. Scott Wilbur, Kent Buhler, Paul H. Lange
Pages 217-242
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In Vivo Experimental Techniques
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Front Matter
Pages 243-243
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- David M. Nanus, Dov Engelstein
Pages 245-253
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- Nobuyasu Nishisaka, Philo Morse, Richard F. Jones, Ching Y. Wang, Gabriel P. Haas
Pages 255-264
About this book
The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.
Reviews
"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal
"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News
"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology
Editors and Affiliations
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Temple University School of Medicine, Philadelphia
Jack H. Mydlo
