Alzheimer's Disease

1. Front Matter

   Pages i-xiv

   PDF

2. Introduction to Alzheimer's Disease

   • David Allsop

   Pages 1-21

3. The Genetics of Alzheimer's Disease

   • Nick Brindle, Peter St. George-Hyslop

   Pages 23-43

4. Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimer's Disease

   • David S. Knopman

   Pages 45-61

5. Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPPα)

   • Steven W. Barger

   Pages 63-78

6. Quantifying Aβ1–40 and Aβ1–42 Using Sandwich-ELISA

   • Daniel M. Skovronsky, Jun Wang, Virginia M.-Y. Lee, Robert W. Doms

   Pages 79-89

7. Electrophoretic Separation and Immunoblotting of Aβ1–40 and Aβ1–42

   • Matthias Staufenbiel, Paolo A. Paganetti

   Pages 91-99

8. Aβ-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes

   • Kurt R. Brunden, June Kocsis-Angle, Paula Embury, Stephen L. Yates

   Pages 101-112

9. Effects of the β-Amyloid Peptide on Membrane Ion Permeability

   • Hugh A. Pearson

   Pages 113-138

10. Analysis of β-Amyloid Peptide Degradation In Vitro

    • Barbara Cordell, Asha Naidu

    Pages 139-148

11. Posttranslational Modifications of Amyloid Precursor Protein

    • Jochen Walter, Christian Haass

    Pages 149-168

12. Posttranslational Modifications of the Amyloid Precursor Protein

    • Chen Liu, Tomasz Rozmyslowicz, Magda Stwora-Wojczyk, Boguslaw Wojczyk, Steven L. Spitalnik

    Pages 169-190

13. Using an Amyloid Precursor Protein (APP) Reporter to Characterize α-Secretase

    • Susan Boseman Roberts

    Pages 191-202

14. Inhibition of α-Secretase by Zinc Metalloproteinase Inhibitors

    • S. Parvathy, Anthony J. Turner, Nigel M. Hooper

    Pages 203-215

15. Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein

    • Carol W. Gray, Eric H. Karran

    Pages 217-228

16. β-Secretase

    • Martin Citron

    Pages 229-238

17. Using β-Secretase Inhibitors to Distiguish the Generation of the Aβ Peptides Terminating at Val-40 and Ala-42

    • Paolo A. Paganetti, Matthias Staufenbiel

    Pages 239-248

18. Designing Animal Models of Alzheimer's Disease with Amyloid Precursor Protein (APP) Transgenes

    • Jeanne F. Loring

    Pages 249-270

19. Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins

    • Toshiharu Suzuki, Kanae Ando, Ko-ichi Iijima, Shinobu Oguchi, Shizu Takeda

    Pages 271-282

20. Determining the Transmembrane Topology of the Presenilins

    • Gopal Thinakaran, Andrew Doan

    Pages 283-296

Alzheimer's disease is the most common cause of senile dementia. Since the discovery in 1984 of the amyloid ?-peptide (A?) as the core protein of the senile plaques present in the brains of Alzheimer's disease sufferers, an immense amount of research has gone into mapping out the molecular basis of this debilitating disease. The aim of Alzheimer's Disease: Methods and Protocols is to bring together the main biochemical, cell biological, and molecular biological techniques and approaches that are being used to investigate the molecular basis of Alzheimer's disease. This volume begins with chapters of an introductory/ review nature. Chapter 1 provides a historical introduction to Alzheimer's d- ease with particular emphasis on the central role played by A? and its re- tion to tau. Chapter 2 examines the genetics underlying this neurodegenerative disease, covering the amyloid precursor protein, apolipoprotein E, and the presenilins. Chapter 3 presents an overview of currently available therapeutic agents and prospects for drugs of the future.

"...this volume contains well-written chapters containing factual insights and useful clarifications. An example is David Allsop's introduction of the field through the orthodox views of the amyloid cascade hypothesis that in the exposition of others is often quite simplistic, but here is presented with complexity and wisdom deserving of serious consideration....they are provided in a format that invites the reader to undertake studies and examine the underpinnings of the subject beyond that found in the primary publications and not covered in such a practical manner in other reviews."- Journal of Molecular Neuroscience

• University of Leeds, Leeds, UK

  Nigel M. Hooper

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