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Rotaviruses

Methods and Protocols

  • Book
  • © 2000

Overview

Editors:
  1. James Gray

    1. Clinical Microbiology and Public Health Laboratory Addenbrooke’s Hospital, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Ulrich Desselberger

    1. Clinical Microbiology and Public Health Laboratory Addenbrooke’s Hospital, Cambridge, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 34)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Rotaviruses: Basic Facts

    • Ulrich Desselberger
    Pages 1-8

  3. Electron Cryomicroscopy and Computer Image Processing Techniques

    • B. V. Venkataram Prasad, Mary K. Estes
    Pages 9-31

  4. Virus Replication

    • John T. Patton, Vladimir Chizhikov, Zenobia Taraporewala, Dayue Chen
    Pages 33-66

  5. Rotavirus Entry into Tissue Culture Cells

    • Joanna M. Gilbert, Harry B. Greenberg
    Pages 67-77

  6. Mixed Infections with Rotaviruses

    • Robert F. Ramig
    Pages 79-99

  7. Pathogenesis and Animal Models

    • Linda J. Saif, Lucy A. Ward
    Pages 101-117

  8. Immunologic Methods and Correlates of Protection

    • Kristine K. Macartney, Paul A. Offit
    Pages 119-132

  9. In Vivo Study of Immunity to Rotaviruses

    • Manuel A. Franco, Harry B. Greenberg
    Pages 133-145

  10. Evaluation of Rotavirus Vaccines in Small Animal Models

    • Max Ciarlet, Margaret E. Conner
    Pages 147-187

  11. Methods of Rotavirus Detection, Sero- and Genotyping, Sequencing, and Phylogenetic Analysis

    • Miren Iturriza Gómara, Jon Green, Jim Gray
    Pages 189-216

  12. Epidemiology of Group A Rotaviruses Surveillance and Burden of Disease Studies

    • Mary Ramsay, David Brown
    Pages 217-238

  13. Future Rotavirus Research

    • Ulrich Desselberger, Mary K. Estes
    Pages 239-258

  14. Back Matter

    Pages 259-262
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About this book

James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality. Presented by experts in the fields of animal and human rotavirus infections and rotavirus vaccine research, these readily reproducible methods detail molecular and other modern techniques, and include relevant background information and various notes to ensure reproducible and robust results. Authoritative and up-to-date, Rotaviruses: Methods and Protocols offers researchers today's benchmark compendium of experimental methods for the investigation of this medically significant virus.

Reviews

"This book will be very useful to both new investigators in the field and persons who have a general interest in rotaviruses and the methods to be used in their study. Many chapter contributors also provide very useful summaries of the methods used in a specific aspect of rotavirus research. These will be helpful to even the most experienced investigator in the field. "-Doody's Health Sciences Book Review Journal

"This book has no recent equivalent....The book will not only be profitable for the RV specialist; people interested in other viruses or cellular physiology will surely benefit from the lessons learned from studies of RV electron cryomicroscopy, RV replication or animal models of RV infections." - Trends in Microbiology

"...methods, which have proven invaluable to studies, are given in detail in this excellent new book. The contributors have written detailed protocols for, predominantly, molecular and serological methods with emphases on those that are somewhat unique to rotavirus research....However, there is much more to this book than the highly detailed protocols....The last of the central chapters is by Mary Ramsay and David Brown and describes the epidemiology of rotavirus infections and concentrates on surveillance and disease burden. Anyone still unconvinced of the need for a rotavirus vaccine should read this chapter."-J. Med. Microbiol

"This high quality compendium comprises 12 concise chapters, each presented in the same easy to follow layout....this book at $89.50 is reasonable value and an essential reference for any laboratory undertaking rotavirus research."- Microbiology Today

Editors and Affiliations

  • Clinical Microbiology and Public Health Laboratory Addenbrooke’s Hospital, Cambridge, UK

    James Gray, Ulrich Desselberger

Bibliographic Information

  • Book Title: Rotaviruses

  • Book Subtitle: Methods and Protocols

  • Editors: James Gray, Ulrich Desselberger

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592590780

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-736-6Published: 17 January 2000

  • Softcover ISBN: 978-1-61737-160-8Published: 09 November 2010

  • eBook ISBN: 978-1-59259-078-0Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: X, 262

  • Topics: Virology

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