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Glycoprotein Methods and Protocols

The Mucins

  • Book
  • © 2000

Overview

Editors:
  1. Anthony P. Corfield

    1. Bristol Royal Infirmary, Bristol, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 125)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Purification of Mucin

    1. Front Matter

      Pages 1-1
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    2. Isolation of Large Gel-Forming Mucins

      • Julia R. Davies, Ingemar Carlstedt
      Pages 3-13

    3. Preparation of Membrane Mucin

      • Kermit L. Carraway
      Pages 15-26

  3. Detection and Ouantitation of Mucin

    1. Front Matter

      Pages 27-27
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    2. Histologically Based Methods for Detection of Mucin

      • Michael D. Walsh, Jeremy R. Jass
      Pages 29-44

    3. Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods

      • Michael A. McGuckin, David J. Thornton
      Pages 45-55

    4. The Gastrointestinal Adherent Mucous Gel Barrier

      • Adrian Allen, Jeffrey P. Pearson
      Pages 57-64

    5. Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling

      • Jan Dekker, B. Van Jan-Willem Klinken, Hans A. Büller, Alexandra W. C. Einerhand
      Pages 65-73

  4. Separation, Identification, and Physical Characterization of Mucins

    1. Front Matter

      Pages 75-75
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    2. Separation and Identification of Mucins and Their Glycoforms

      • David J. Thornton, Nagma Khan, John K. Sheehan
      Pages 77-85

    3. Heterogeneity and Size Distribution of Gel-Forming Mucins

      • John K. Sheehan, David J. Thornton
      Pages 87-96

  5. Rheology of Mucin

    1. Front Matter

      Pages 97-97
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    2. Rheology of Mucin

      • Jeffrey P. Pearson, Adrian Allen, David A. Hutton
      Pages 99-109

  6. Mucin Peptide Analysis

    1. Front Matter

      Pages 111-111
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    2. Amino Acid Analysis of Mucins

      • Jun X. Yan, Nicolle H. Packer
      Pages 113-119

    3. Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation

      • Natasha E. Zachara, Andrew A. Gooley
      Pages 121-128

    4. Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies

      • Andrea Murray, Deirdre A. O’Sullivan, Michael R. Price
      Pages 129-141

    5. Mucin Domains to Explore Disulfide-Dependent Dimer Formation

      • Sherilyn L. Bell, Janet F. Forstner
      Pages 143-155

  7. Carbohydrate Structural Analysis of Mucins

    1. Front Matter

      Pages 157-157
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About this book

The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.

Reviews

"There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included." -Microbiology Today

Editors and Affiliations

  • Bristol Royal Infirmary, Bristol, UK

    Anthony P. Corfield

Bibliographic Information

  • Book Title: Glycoprotein Methods and Protocols

  • Book Subtitle: The Mucins

  • Editors: Anthony P. Corfield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590489

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-720-5Published: 01 March 2000

  • Softcover ISBN: 978-1-61737-149-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-048-3Published: 26 October 2007

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 506

  • Topics: Biochemistry, general

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