Overview
- Editors:
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Martin J. Tymms
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National Vision Research Institute of Australia, Carlton, Australia
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Ismail Kola
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Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Australia
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Table of contents (25 protocols)
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- Paul J. Hertzog, Ismail Kola
Pages 1-10
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- Vivienne S. Marshall, Michelle A. Waknitz, James A. Thomson
Pages 11-18
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- Louise D. Barnett, Frank Köntgen
Pages 65-82
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- Trevor J. Wilson, Ismail Kola
Pages 83-94
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- Mireille H. Lahoud, Trevor J. Wilson, Paul J. Hertzog
Pages 107-119
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- Angelika Bonin, Susan W. Reid, Lino Tessarollo
Pages 121-134
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- Mika Tanaka, Anna-Katerina Hadjantonakis, Andras Nagy
Pages 135-154
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- Ivan Bertoncello, Brenda Williams
Pages 181-203
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- L. Philip Sanford, Suhas Kallapur, Ilona Ormsby, Thomas Doetschman
Pages 217-225
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- Gabriel A. Quinlan, Bruce P. Davidson, Patrick P. L. Tam
Pages 227-250
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- Hein te Riele, Conny Brouwers, Marleen Dekker
Pages 251-262
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- Anna M. Wobus, Kaomei Guan, Uta Pich
Pages 263-286
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- Francesca Lazner, Ismail Kola, Elizabeth Stadler, Andrew G. Elefanty
Pages 287-300
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- Linda M. Whyatt, Peter D. Rathjen
Pages 301-318
About this book
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Reviews
"This is a valuable reference work, which will no doubt find a home on many lab benches." -E-STREAMS
Editors and Affiliations
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National Vision Research Institute of Australia, Carlton, Australia
Martin J. Tymms
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Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Australia
Ismail Kola
