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In Vitro Neurochemical Techniques

  • Book
  • © 1999

Overview

Editors:
  1. Alan A. Boulton

    1. Unlversity of Saskatchewan, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Glen B. Baker

    1. Unlversity of Alberta, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Alan N. Bateson

    1. Unlversity of Alberta, Canada

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Neuromethods (NM, volume 34)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Characterization of Receptors by Radiolabeled Ligand-Binding Techniques

    • Martin Davies, Susan M. j. Dunn
    Pages 1-35

  3. Electrophysiological Studies on Receptors In Vitro

    • Qi-Ying Liu, Anne E. Schaflner, jeffery L. Barker
    Pages 37-61

  4. Subtractive Hybridization

    • H. Michael Tucker, Steven Estus
    Pages 63-73

  5. Messenger RNA Differential Display

    • Véronique A.-M.I. Tanay, Alan N. Bateson
    Pages 75-86

  6. Localization of mRNAs in Brain Sections by in situ Hybridization Using Oligonucleotide Probes

    • Alan N. Bateson
    Pages 87-99

  7. Multiprobe Oligonucleotide Solution Hybridization for the Determination of Relative mRNA Levels

    • Alan N. Bateson, Vékonique A.-M.I. Tanay
    Pages 101-126

  8. Competitive RT-PCR to Quantitate Steady-State mRNA Levels

    • Dennis R. Grayson, Snezana Ikonomovic
    Pages 127-151

  9. A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes

    • A. Petronis, A. Timinskas, V. Basile, A. Vicente, A. Janulaitis, J. L. Kennedy
    Pages 153-166

  10. Western Blot Analysis

    • D. D. Mousseau, V. L. Raghavendra Rao
    Pages 167-191

  11. The Phosphoinositide Signal Transduction System

    • Richard S. Jope, Xiaohua Li, Mary A. Pacheco
    Pages 193-215

  12. Protein Phosphorylation

    • Gail V. W. Johnson, Penny K. Davis
    Pages 217-256

  13. Immunoprecipitation of Receptors

    • Paul L. Chazot, Simon Pollard, F. Anne Stephenson
    Pages 257-285

  14. Back Matter

    Pages 287-296
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About this book

In Vitro Neurochemical Techniques is the third work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The key techniques detailed in this new edition encompass the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover the measurement of changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways.
The companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, cover both in vivo methods and in vitro cell neurobiology approaches. Like the original, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.





Editors and Affiliations

  • Unlversity of Saskatchewan, Canada

    Alan A. Boulton

  • Unlversity of Alberta, Canada

    Glen B. Baker, Alan N. Bateson

Bibliographic Information

  • Book Title: In Vitro Neurochemical Techniques

  • Editors: Alan A. Boulton, Glen B. Baker, Alan N. Bateson

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1385/0896035093

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Hardcover ISBN: 978-0-89603-509-6Published: 06 November 1998

  • Softcover ISBN: 978-1-61737-055-7Published: 09 November 2010

  • eBook ISBN: 978-1-59259-639-3Published: 06 February 2008

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 296

  • Topics: Neurosciences

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