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Lectin Methods and Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Jonathan M. Rhodes

    1. University of Liverpool, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Jeremy D. Milton

    1. University of Liverpool, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 9)

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Table of contents (49 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Lectin Histochemistry and Cytochemistry

    1. Front Matter

      Pages 1-1
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    2. Light Microscopy

      • Anthony J. Leathem, Susan A. Brooks
      Pages 3-20

    3. Lectin Histochemistry and Cytochemistry—Light Microscopy

      • Robert W. Stoddart, Carolyn J. P. Jones
      Pages 21-39

    4. Lectin—Gold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins

      • Jürgen Roth, Christian Zuber, Tetsutaro Sata, Wei-Ping Li
      Pages 41-53

    5. Use of Fluorochrome-Labeled Lectins in Light Microscopy

      • Udo Schumacher, Barry S. Mitchell
      Pages 55-64

    6. The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage

      • Sibylle Hoebt-Schmidt
      Pages 65-71

    7. Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis

      • Timothy Richard Helliwell
      Pages 73-94

    8. Electron Microscopy

      • Adi Ellinger
      Pages 97-110

    9. Electron Microscopy

      • Rainer Herken, Berti Manshausen
      Pages 111-119

    10. Amplification of Lectin-Gold Histochemistry

      • Juan F. Madrld, Francisco Hernández, José Ballesta
      Pages 121-132

    11. Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines

      • Barry S. Mitchell, Udo Schumacher
      Pages 133-143

  3. Use of Lectins for Structural Analysis of Oligosaccharide Chains

    1. Front Matter

      Pages 145-145
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    2. Application of Sequential Smith Degradation to Lectin Blots

      • Chi Kong Ching
      Pages 147-157

    3. Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues

      • Christian Zuber, Wei-Ping Li, Jürgen Roth
      Pages 159-166

    4. Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis

      • Gregers J. Gram, John-Erik Stig Hansen
      Pages 167-174

    5. Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins

      • Sigvard Olofsson, Anders Bolmstedt
      Pages 175-192

  4. Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins

    1. Front Matter

      Pages 193-193
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    2. Use of Lectin for Detection of Agalactosyl IgG

      • Naoyuki Tsuchiya, Tamao Endo, Naohisa Kochibe, Koji Ito, Akira Kobata
      Pages 195-205

    3. Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins

      • Yutaka Aoyagi, Hitoshi Asakura
      Pages 207-214
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About this book

Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology

Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. The book will immediately become the standard practical resource and guide to lectin techniques for today's biomedical researchers.

Editors and Affiliations

  • University of Liverpool, UK

    Jonathan M. Rhodes, Jeremy D. Milton

Bibliographic Information

  • Book Title: Lectin Methods and Protocols

  • Editors: Jonathan M. Rhodes, Jeremy D. Milton

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896033961

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-396-2Published: 23 September 1997

  • Softcover ISBN: 978-1-61737-024-3Published: 09 November 2010

  • eBook ISBN: 978-1-59259-593-8Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVIII, 616

  • Topics: Biochemistry, general

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