Photosynthesis Research Protocols

1. Front Matter

   Pages i-xiv

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2. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins from Higher Plants

   • Yasusi Yamamoto, Jing Leng, Jian-Ren Shen

   Pages 1-10

3. Isolation of Photosystem I Submembrane Fractions

   • Johanne Harnois, Najoua Msilini, Robert Carpentier

   Pages 11-15

4. Isolation of Photosystem II Reaction Center Complexes from Plants

   • Michael Seibert, Rafael Picorel

   Pages 17-27

5. Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803

   • Dmitrii V. Vavilin

   Pages 29-40

6. Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria

   • Jian-Ren Shen, Keisuke Kawakami, Hiroyuki Koike

   Pages 41-51

7. Isolation of Cytochrome b ₆ f Complex from Grana and Stroma Membranes from Spinach Chloroplasts

   • Elżbieta Romanowska

   Pages 53-64

8. Purification and Crystallization of the Cyanobacterial Cytochrome b ₆ f Complex

   • Danas Baniulis, Huamin Zhang, Taisiya Zakharova, S. Saif Hasan, William A. Cramer

   Pages 65-77

9. Purification of Plastocyanin and Cytochrome c ₆ from Plants, Green Algae, and Cyanobacteria

   • José A. Navarro, Manuel Hervás, Miguel A. De la Rosa

   Pages 79-94

10. Isolation and Identification of Chloroplast Lipids

    • Norihiro Sato, Mikio Tsuzuki

    Pages 95-104

11. Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants

    • Rafael Picorel, Miguel Alfonso, Michael Seibert

    Pages 105-112

12. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex

    • Wolfgang Rühle, Harald Paulsen

    Pages 113-125

13. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-assemblies with Light-Inducible Structural Transitions

    • Ilian Simidjiev, Zsuzsanna Várkonyi, Petar H. Lambrev, Győző Garab

    Pages 127-138

14. Protein Targeting Across and into Chloroplast Membranes

    • Shari M. Lo, Steven M. Theg

    Pages 139-157

15. Proteomic Analysis of Thylakoid Membranes

    • Venkateswarlu Yadavalli, Sreedhar Nellaepalli, Rajagopal Subramanyam

    Pages 159-170

16. Thylakoid Phosphoproteins: Identification of Phosphorylation Sites

    • Anne Rokka, Eva-Mari Aro, Alexander V. Vener

    Pages 171-186

17. Direct Detection of Free Radicals and Reactive Oxygen Species in Thylakoids

    • Éva Hideg, Tamás Kálai, Kálmán Hideg

    Pages 187-200

18. Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher Plants

    • Nobuyoshi Nijo, Björn Lundin, Miho Yoshioka, Noriko Morita, Yasusi Yamamoto

    Pages 201-215

19. Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure–Function Characterization

    • David Joly, Sridharan Govindachary, Mário Fragata

    Pages 217-245

20. Physical and Chemical Immobilization Methods of Photosynthetic Materials

    • Lise Barthelmebs, Robert Carpentier, Régis Rouillon

    Pages 247-256

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.

• Biomass
• Isolation, treatment, & analysis
• Photosynthetic carbon fixation
• Plant bioenergetics
• Plant physiology
• Plant productivity

From the reviews of the second edition:

“Photosynthesis research protocols focuses on various aspects of photosynthesis research and its chapters reflect the whole complexity of this research field. … All chapters are of high quality and written by leading scientists in their fields. Illustrations are provided when needed and each protocol is sufficiently detailed also to allow access to beginners in the field. … every photosynthesis researcher will surely find several chapters of interest to them within this book.” (Dario Leister, Annals of Botany, Vol. 112 (1), 2013)

“This book represents a collection of materials and methods that are highly useful for researchers in the field of photosynthesis. … various chapters have been revised and the literature has been updated. … every lab working in the field of photosynthesis should have at least one copy. The protocols described in the various chapters are written in a concise and understandable way.” (Reimund Goss, Journal of Plant Physiology, Vol. 169, 2012)

“This book presents a set of protocols focused to the study of photosynthesis from multiple points of view. … new chapters have been added to this edition dealing with the isolation, quantification of Rubisco and Rubisco activase. All the contributors are experienced scientists on the analyzed subjects. The structure of each chapter … enhances the value of the information. In conclusion, this book is a fundamental reference for plant researchers, both for the wide spectrum of subjects covered and the simplicity of the layout.” (Alejandra Landau and Noemí Colombo, Boletin de Bibleoteca del IMYZA-INTA, Issue 26, June, 2011)

• , Département de Chimie Biologie, Université du Québec à Trois-Rivières, Trois-Rivieres, Canada

  Robert Carpentier

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