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Cell-Penetrating Peptides

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Ülo Langel

    1. , Department of Neurochemistry, Stockholm University, Stockholm, Sweden

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  • Fully updated cutting-edge methods for studies of mechanisms and applications of cell-penetrating peptides
  • Includes methods for preparation and analysis of cellular uptake of cell-penetrating peptides and cargos
  • Provides description of studies for cellular uptake, toxicity and bioeffects of cell-penetratin peptide-assisted delivery
  • Provides description of in vivo studies of CPP-assisted delivery
  • Provides description of design synthesis methods of oligonucleotides
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 683)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xv
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Classes and Prediction of Cell-Penetrating Peptides

      • Maria Lindgren, Ülo Langel
      Pages 3-19

    3. Penetratin Story: An Overview

      • Edmond Dupont, Alain Prochiantz, Alain Joliot
      Pages 21-29

  3. Methods to Test Mechanisms of Cell-Penetrating Peptides

    1. Front Matter

      Pages 31-31
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  4. Methods to test mechanisms of cell-penetrating peptides

    1. Testing Membrane Interactions of CPPs

      • Astrid Gräslund, Lena Mäler
      Pages 33-40

    2. Interactions of Amphipathic CPPs with Model Membranes

      • Sébastien Deshayes, Karidia Konate, Gudrun Aldrian, Frédéric Heitz, Gilles Divita
      Pages 41-56

    3. NMR Studies of Three-Dimensional Structure and Positioning of CPPs in Membrane Model Systems

      • Lena Mäler, Astrid Gräslund
      Pages 57-67

    4. Measurements of the Intracellular Stability of CPPs

      • Ivo R. Ruttekolk, Wouter P. R. Verdurmen, Yi-Da Chung, Roland Brock
      Pages 69-80

    5. Tools for Predicting Binding and Insertion of CPPs into Lipid Bilayers

      • Paulo F. Almeida
      Pages 81-98

    6. Studies of Proteoglycan Involvement in CPP-Mediated Delivery

      • Anders Wittrup, Si-He Zhang, Mattias Belting
      Pages 99-115

    7. Uptake Kinetics of Cell-Penetrating Peptides

      • Anders Florén, Imre Mäger, Ülo Langel
      Pages 117-128

    8. Thermodynamics of Lipid Interactions with Cell-Penetrating Peptides

      • Reto Sauder, Joachim Seelig, André Ziegler
      Pages 129-155

    9. Calcium and Membrane Repair

      • Caroline Palm-Apergi, Mattias Hällbrink
      Pages 157-164

    10. Mapping of Protein Transduction Pathways with Fluorescent Microscopy

      • Helin Räägel, Pille Säälik, Ülo Langel, Margus Pooga
      Pages 165-179

    11. Insight into Cell-Entry Mechanisms of CPPs by Electron Microscopy

      • Kärt Padari, Annely Lorents, Eija Jokitalo, Margus Pooga
      Pages 181-193

    12. Toxicity Methods for CPPs

      • Per Lundin, Samir EL Andaloussi, Ülo Langel
      Pages 195-205

    13. Comparison of CPP Uptake Methods

      • Tina Holm, Samir EL Andaloussi, Ülo Langel
      Pages 207-217

    14. Characterization of Cellular Internalization Pathways for CPP-Mediated Oligonucleotide Delivery

      • Peter Guterstam, Samir EL Andaloussi, Ülo Langel
      Pages 219-230

  5. Functionality and Cell-Penetrating Peptides

    1. Front Matter

      Pages 231-231
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Keywords

About this book

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.

Reviews

From the reviews:

“Prof. Ulo Langel … rightly explain in a necessary preface that there are in use so many definition for the same things: protein transduction domain (PTDs), Trojan peptides, model amphipathic peptides (MAPs), membrane translocating sequences (MTS) that the best way to refer to all of these molecules is to call all of them cell-penetrating peptides, CPPs. … Clearly enough, the cell-penetrating peptides volume is surely of great help for basic research and for clinicians engaged in translational medicine.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Editors and Affiliations

  • , Department of Neurochemistry, Stockholm University, Stockholm, Sweden

    Ülo Langel

Bibliographic Information

  • Book Title: Cell-Penetrating Peptides

  • Book Subtitle: Methods and Protocols

  • Editors: Ülo Langel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-919-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60761-918-5Published: 08 November 2010

  • Softcover ISBN: 978-1-4939-6155-9Published: 23 August 2016

  • eBook ISBN: 978-1-60761-919-2Published: 05 November 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 586

  • Topics: Cell Biology

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