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Enzyme Stabilization and Immobilization

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Shelley D. Minteer

    1. Department of Chemistry, St. Louis University, St. Louis, USA

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  • Provides protocols for enzyme stabilization that can be used for any enzyme of interest to the reader
  • Includes up-to-date protocols on the latest enzyme immobilization techniques
  • Provides a thorough review of methods for accurately determining enzyme activity and stability
  • Procedures are scaleable from microscale molecular biology assays to industrial bioprocessing
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 679)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to the Field of Enzyme Immobilization and Stabilization

    • Michael J. Moehlenbrock, Shelley D. Minteer
    Pages 1-7

  3. Stabilization of Enzymes Through Encapsulation in Liposomes

    • Makoto Yoshimoto
    Pages 9-18

  4. Micellar Enzymology for Thermal, pH, and Solvent Stability

    • Shelley D. Minteer
    Pages 19-24

  5. Lipase Activation and Stabilization in Room-Temperature Ionic Liquids

    • Joel L. Kaar
    Pages 25-35

  6. Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems

    • Andreas Buthe, Songtao Wu, Ping Wang
    Pages 37-48

  7. Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

    • Allan E. David, Arthur J. Yang, Nam Sun Wang
    Pages 49-66

  8. Nanoporous Gold for Enzyme Immobilization

    • Keith J. Stine, Kenise Jefferson, Olga V. Shulga
    Pages 67-83

  9. Enzyme Stabilization via Bio-templated Silicification Reactions

    • Glenn R. Johnson, Heather R. Luckarift
    Pages 85-97

  10. The Immobilization of Enzyme on Eupergit® Supports by Covalent Attachment

    • Zorica D. Knežević-Jugović, Dejan I. Bezbradica, Dušan Ž. Mijin, Mirjana G. Antov
    Pages 99-111

  11. Micellar Polymer Encapsulation of Enzymes

    • Sabina Besic, Shelley D. Minteer
    Pages 113-131

  12. Enzyme Stabilization via Cross-Linked Enzyme Aggregates

    • Munishwar N. Gupta, Smita Raghava
    Pages 133-145

  13. Enzyme Immobilization in Polyelectrolyte Microcapsules

    • Michael J. McShane
    Pages 147-154

  14. Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

    • Nenad B. Milosavić, Radivoje M. Prodanović
    Pages 155-163

  15. Enzyme–Nanoparticle Conjugates for Biomedical Applications

    • Alexey A. Vertegel, Vladimir Reukov, Victor Maximov
    Pages 165-182

  16. Enzyme Nanoparticle Fabrication: Magnetic Nanoparticle Synthesis and Enzyme Immobilization

    • Patrick A. Johnson, Hee Joon Park, Ashley J. Driscoll
    Pages 183-191

  17. Cytochrome c Stabilization and Immobilization in Aerogels

    • Amanda S. Harper-Leatherman, Jean Marie Wallace, Debra R. Rolison
    Pages 193-205

  18. Kinetic Measurements for Enzyme Immobilization

    • Michael J. Cooney
    Pages 207-225

  19. Back Matter

    Pages 227-229
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Keywords

About this book

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

Editors and Affiliations

  • Department of Chemistry, St. Louis University, St. Louis, USA

    Shelley D. Minteer

Bibliographic Information

  • Book Title: Enzyme Stabilization and Immobilization

  • Book Subtitle: Methods and Protocols

  • Editors: Shelley D. Minteer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-895-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60761-894-2Published: 29 September 2010

  • Softcover ISBN: 978-1-4939-5792-7Published: 23 August 2016

  • eBook ISBN: 978-1-60761-895-9Published: 24 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 229

  • Topics: Biochemistry, general

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