Suppression and Regulation of Immune Responses

1. Front Matter

   Pages i-xviii

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2. Regulatory Cells

   1. Front Matter

      Pages 1-1

      PDF

   2. Natural and Induced T CD4+CD25+FOXP3+ Regulatory T Cells

      • Lucienne Chatenoud

      Pages 3-13

   3. Polyclonal Expansion of Human CD4+CD25+ Regulatory T Cells

      • Petra Hoffmann, Ruediger Eder, Matthias Edinger

      Pages 15-30

   4. Methods for In Vitro Generation of Human Type 1 Regulatory T Cells

      • Silvia Gregori, Maria Grazia Roncarolo, Rosa Bacchetta

      Pages 31-46

   5. Ex Vivo Generation of Regulatory T Cells: Characterization and Therapeutic Evaluation in a Model of Chronic Colitis

      • Fridrik Karlsson, Sherry A. Robinson-Jackson, Laura Gray, Songlin Zhang, Matthew B. Grisham

      Pages 47-61

   6. Phenotypic and Functional Characterization of CD8+ T Regulatory Cells

      • Séverine Ménoret, Carole Guillonneau, Séverine Bezié, Lise Caron, Ignacio Anegon, Xian-Liang Li

      Pages 63-83

   7. Regulatory CD4– CD8– Double Negative T Cells

      • Edward Y. Kim, Stephen C. Juvet, Li Zhang

      Pages 85-98

   8. Identifying Regulatory B Cells (B10 Cells) that Produce IL-10 in Mice

      • Takashi Matsushita, Thomas F. Tedder

      Pages 99-111

   9. DCs in Immune Tolerance in Steady-State Conditions

      • Tomohiro Fukaya, Hideaki Takagi, Honami Taya, Katsuaki Sato

      Pages 113-126

   10. Plasmacytoid Dendritic Cells in Tolerance

       • Eric Gehrie, William Van der Touw, Jonathan S. Bromberg, Jordi C. Ochando

       Pages 127-147

   11. In Vitro-Generated DC with Tolerogenic Functions: Perspectives for In Vivo Cellular Therapy

       • Cees van Kooten, Kyra A. Gelderman

       Pages 149-159

   12. Preparation of Mouse Bone Marrow-Derived Dendritic Cells with Immunoregulatory Properties

       • Mercedes Segovia, Maria Cristina Cuturi, Marcelo Hill

       Pages 161-168

   13. Myeloid-Derived Suppressor Cells: Characterization and Expansion in Models of Endotoxemia and Transplantation

       • Nicolas Van Rompaey, Alain Le Moine

       Pages 169-180

   14. Human Regulatory Macrophages

       • James A. Hutchinson, Paloma Riquelme, Edward K. Geissler, Fred Fändrich

       Pages 181-192

   15. NKT and Tolerance

       • Julien Diana, Lucie Beaudoin, Anne-Sophie Gautron, Agnès Lehuen

       Pages 193-206

   16. Antiinflammatory and Immunosuppressive Functions of Mast Cells

       • Janet Kalesnikoff, Stephen J. Galli

       Pages 207-220

   17. A Mesenchymal Stem Cell Potency Assay

       • Joy Jiao, Jack M. Milwid, Martin L. Yarmush, Biju Parekkadan

       Pages 221-231

   18. In Vitro Analyses of the Immunosuppressive Properties of Neural Stem/Progenitor Cells Using Anti-CD3/CD28-Activated T Cells

       • Virginie Bonnamain, Isabelle Neveu, Philippe Naveilhan

       Pages 233-243

3. Regulatory Molecules

   1. Front Matter

      Pages 245-245

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Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation. In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins such as B, NK, myeloid, and dendritic cells, as well as the role of several suppressive molecules in immunoregulation. Particular emphasis is placed on the characterization of the molecular mechanisms and the therapeutic applications of regulatory cells and molecules in human diseases. Written as a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Comprehensive and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols serves as a key reference for scientists seeking a way toward greater control over the enormous power of the complex and vital immune system.

• Immunoregulation
• Molecular mechanisms
• Physiopathological situations
• Regulatory cells
• Suppressive molecules
• T-cells
• Therapeutic applications

From the reviews:

“This is a highly informative book, packed with a wealth of information covering all aspects of immune suppression and regulatory mechanisms. There is a diverse array of protocols offered, which are generally clear and easy to follow. … the wealth of information and literature reviews included make this book worth purchasing for those working in this field.” (Andrew Vaughan, Immunology News, August, 2011)

• CHUHotel Dieu, INSERM UMR 643, Nantes, France

  Maria Cristina Cuturi

• CHU Hotel Dieu, INSERM UMR 643, Nantes, France

  Ignacio Anegon

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