Small Molecule Microarrays

1. Front Matter

   Pages i-xi

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2. The Expanding World of Small Molecule Microarrays

   • Mahesh Uttamchandani, Shao Q. Yao

   Pages 1-15

3. Construction of Photo-Cross-Linked Microarrays of Small Molecules

   • Naoki Kanoh

   Pages 17-22

4. Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules

   • Hyang Yeon Lee, Seung Bum Park

   Pages 23-42

5. Detecting Protein–Small Molecule Interactions Using Fluorous Small-Molecule Microarrays

   • Arturo J. Vegas, Angela N. Koehler

   Pages 43-55

6. A Method for Small Molecule Microarray-Based Screening for the Rapid Discovery of Affinity-Based Probes

   • Haibin Shi, Mahesh Uttamchandani, Shao Q. Yao

   Pages 57-68

7. Nanodroplet Chemical Microarrays and Label-Free Assays

   • Dhaval Gosalia, Scott L. Diamond

   Pages 69-78

8. Nanodroplet Microarrays for High-Throughput Enzyme Screening

   • Kang L. D. Aw, Shao Q. Yao, Mahesh Uttamchandani

   Pages 79-93

9. The Application of the Chemical Array for Biological Study

   • Isao Miyazaki, Siro Simizu, Hiroyuki Osada

   Pages 95-107

10. Peptide Arrays with a Chip

    • Alexander Nesterov, Edgar Dörsam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Märkle, Felix Löffler et al.

    Pages 109-124

11. In Situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon Nanowires

    • Gaëlle Piret, Hervé Drobecq, Rabah Boukherroub, Oleg Melnyk

    Pages 125-133

12. In Situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens

    • Rémi Desmet, Eric Diesis, Hervé Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie et al.

    Pages 135-145

13. Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection

    • Marina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna et al.

    Pages 147-160

14. Antibody Signatures Defined by High-Content Peptide Microarray Analysis

    • Antonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, Mike Schutkowski

    Pages 161-172

15. High Density Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysates

    • Alexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, Mike Schutkowski

    Pages 173-181

16. A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates

    • Xiaoming Han, Yoshiki Katayama

    Pages 183-194

17. Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications

    • Sungjin Park, Myung-Ryul Lee, Injae Shin

    Pages 195-208

18. A Microarray-Based Method to Perform Nucleic Acid Selections

    • Olga Aminova, Matthew D. Disney

    Pages 209-224

19. Back Matter

    Pages 225-228

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Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

• Array fabrication
• Carbohydrates
• Chemical and peptide libraries
• Enzyme profiling
• Hydra
• Inhibitor discovery
• Library synthesis
• Lysate protein screening
• Microarray
• Molecular immobilization
• SMM
• enzymes
• molecular biology
• microarrays

• , DSO National Laboratories, Defence Medical and Environmental Resear, Singapore, Singapore

  Mahesh Uttamchandani

• Faculty of Science, Depts. Chemistry & Biological Sciences, National University of Singapore, Singapore, Singapore

  Shao Q. Yao

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