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Mouse Molecular Embryology

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Mark Lewandoski

    1. Center, National Cancer Institute Frederick Cancer Research & Development, Frederick, USA

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  • Present cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1092)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. In Situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional β-Galactosidase Staining

    • Yoshihiro Komatsu, Satoshi Kishigami, Yuji Mishina
    Pages 1-15

  3. Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos

    • Kristin K. Biris, Terry P. Yamaguchi
    Pages 17-30

  4. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections

    • Davide De Pietri Tonelli, Yoanne M. Clovis, Wieland B. Huttner
    Pages 31-42

  5. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays

    • Latasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar, Joyce A. Lloyd
    Pages 43-60

  6. EMAGE: Electronic Mouse Atlas of Gene Expression

    • Lorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman, Yiya Yang, Nick Burton, Jianguo Rao et al.
    Pages 61-79

  7. Real-Time PCR Quantification of Gene Expression in Embryonic Mouse Tissue

    • Eric Villalon, David J. Schulz, Samuel T. Waters
    Pages 81-94

  8. Identifying Essential Genes in Mouse Development via an ENU-Based Forward Genetic Approach

    • Aimin Liu, Jonathan Eggenschwiler
    Pages 95-118

  9. Generation of Mouse Embryos with Small Hairpin RNA-Mediated Knockdown of Gene Expression

    • David A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert, Patrick P. L. Tam
    Pages 119-142

  10. Generation of Tissue Organoids by Compaction Reaggregation

    • Julie M. Sheridan, C. Clare Blackburn
    Pages 143-151

  11. Ultrarapid Vitrification of Mouse Oocytes and Embryos

    • Mark G. Larman, David K. Gardner
    Pages 153-165

  12. Mammalian Preimplantation Embryo Culture

    • David K. Gardner, Michelle Lane
    Pages 167-182

  13. Serum-Free Culture of Mid-gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs

    • Julie Gordon, Billie A. Moore, C. Clare Blackburn, Nancy R. Manley
    Pages 183-194

  14. Genetically Encoded Probes Provide a Window on Embryonic Arrhythmia

    • Yvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran et al.
    Pages 195-219

  15. Microscopic Computed Tomography-Based Skeletal Phenotyping for Genetic Model Organisms

    • Suresh I. Prajapati, Lisa Nevell, Charles Keller
    Pages 221-226

  16. Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos

    • Daisuke Sakai, Paul A. Trainor
    Pages 227-234

  17. Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues

    • Kaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi et al.
    Pages 235-253

  18. Protein/Peptide Transduction in Metanephric Explant Culture

    • Sergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, Alan O. Perantoni
    Pages 255-267

  19. Detection of Cells Programmed to Die in Mouse Embryos

    • Rocío Hernández-Martínez, Rodrigo Cuervo, Luis Covarrubias
    Pages 269-289

  20. Microscopic Computed Tomography-Based Virtual Histology of Embryos

    • Suresh I. Prajapati, David R. Rodriguez, Charles Keller
    Pages 291-296
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Keywords

About this book

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study  mouse embryology.  These include protocols and techniques that  are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.  With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

  

Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

Editors and Affiliations

  • Center, National Cancer Institute Frederick Cancer Research & Development, Frederick, USA

    Mark Lewandoski

Bibliographic Information

  • Book Title: Mouse Molecular Embryology

  • Book Subtitle: Methods and Protocols

  • Editors: Mark Lewandoski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-292-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-60327-290-2Published: 07 December 2013

  • Softcover ISBN: 978-1-4899-7773-1Published: 23 August 2016

  • eBook ISBN: 978-1-60327-292-6Published: 11 December 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 465

  • Number of Illustrations: 36 b/w illustrations, 71 illustrations in colour

  • Topics: Cell Biology, Embryology

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