Editors:
- Comprised of twenty-seven chapters contributed by world-renowned experts in the field
- Fully updated from the first edition to offer cutting-edge methods for the rapid detection of RONs and redox stress
- Includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 610)
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Table of contents (27 protocols)
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Front Matter
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Reactive Oxygen and Nitrogen Species
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Front Matter
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Natural and Synthetic Antioxidants
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Front Matter
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Cellular Oxidative Stress
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Front Matter
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About this book
In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
Reviews
From the reviews of the second edition:
“The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. … Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models.” (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)Editors and Affiliations
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A& M College, Southern University &, Baton Rouge, U.S.A.
Rao M. Uppu
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School of Medicine, Tulane University, New Orleans, U.S.A.
Subramanyam N. Murthy
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Biodynamics Inst., Louisiana State University, Baton Rouge, U.S.A.
William A. Pryor
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Dorothy M. Davis Heart &, Ohio State University, Columbus, U.S.A.
Narasimham L. Parinandi
Bibliographic Information
Book Title: Free Radicals and Antioxidant Protocols
Editors: Rao M. Uppu, Subramanyam N. Murthy, William A. Pryor, Narasimham L. Parinandi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-029-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-58829-710-5Published: 23 December 2009
Softcover ISBN: 978-1-61779-683-8Published: 06 April 2012
eBook ISBN: 978-1-60327-029-8Published: 15 December 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 480
Number of Illustrations: 104 b/w illustrations, 11 illustrations in colour
Additional Information: Originally published as volume 108 in the series: Methods Molecular Biology
Topics: Biochemistry, general, Analytical Chemistry, Oxidative Stress