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Histidine Phosphorylation

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2077)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation

    • Jonathan T. Fischer, Ilana Heckler, Elizabeth M. Boon
    Pages 37-49
  3. Analysis of 1- and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria

    • Alice K. M. Clubbs Coldron, Dominic P. Byrne, Patrick A. Eyers
    Pages 63-81
  4. Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry

    • Lauren J. Tomlinson, Alice K. M. Clubbs Coldron, Patrick A. Eyers, Claire E. Eyers
    Pages 83-91
  5. In Vitro Assays for Measuring Protein Histidine Phosphatase Activity

    • Brandon S. McCullough, Amy M. Barrios
    Pages 109-120
  6. Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases

    • Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, Patricia Casino
    Pages 121-140
  7. Manipulation of Bacterial Signaling Using Engineered Histidine Kinases

    • Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers
    Pages 141-163
  8. Development of a Light-Dependent Protein Histidine Kinase

    • Aleksandra E. Bury, Klaas J. Hellingwerf
    Pages 165-180
  9. Proteome Bioinformatics Methods for Studying Histidine Phosphorylation

    • Andrew R. Jones, Oscar Martin Camacho
    Pages 237-250
  10. Back Matter

    Pages 251-256

About this book

This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field. 

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Editors and Affiliations

  • Department of Biochemistry, Centre for Proteome Research Institute of Integrative Biology, University of Liverpool, Liverpool, UK

    Claire E. Eyers

About the editor

 

Bibliographic Information

  • Book Title: Histidine Phosphorylation

  • Book Subtitle: Methods and Protocols

  • Editors: Claire E. Eyers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9884-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9883-8Published: 10 November 2019

  • Softcover ISBN: 978-1-4939-9886-9Published: 10 November 2020

  • eBook ISBN: 978-1-4939-9884-5Published: 09 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 256

  • Number of Illustrations: 10 b/w illustrations, 44 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access