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Cryptosporidium

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Jan R. Mead

    1. Atlanta Veterans Affairs Medical Center, Decatur, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Michael J. Arrowood

    1. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2052)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cryptosporidium Diagnostic Assays: Microscopy

    • Guy Robinson, Rachel M. Chalmers
    Pages 1-10

  3. Cryptosporidium Diagnostic Assays: Molecular Detection

    • Guy Robinson, Kristin Elwin, Rachel M. Chalmers
    Pages 11-22

  4. Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

    • Amy M. Kahler, Vincent R. Hill
    Pages 23-41

  5. Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation

    • Michael J. Arrowood
    Pages 43-59

  6. Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

    • Jeffrey W. Priest, Delynn M. Moss
    Pages 61-85

  7. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

    • Iwona Driskell, Roberta M. O’Connor
    Pages 87-102

  8. Cryptosporidium Genotyping for Epidemiology Tracking

    • Dawn M. Roellig, Lihua Xiao
    Pages 103-116

  9. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection

    • Anson V. Koehler, Jan Šlapeta
    Pages 117-127

  10. Preparation of Cryptosporidium DNA for Whole Genome Sequencing

    • Guy Robinson, Rachel M. Chalmers
    Pages 129-138

  11. Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org

    • Susanne Warrenfeltz, Jessica C. Kissinger, On Behalf of the EuPathDB Team
    Pages 139-192Open Access

  12. A Novel Method to Silence Genes in Cryptosporidium

    • Alejandro Castellanos-Gonzalez
    Pages 193-203

  13. Use of miRNAs to Study Host Cell–Parasite Interactions

    • Ji-Hao Xu, Nicholas W. Mathy, Xian-Ming Chen
    Pages 205-218

  14. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

    • Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen
    Pages 219-228

  15. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

    • Karine Sonzogni-Desautels, Jan R. Mead, Momar Ndao
    Pages 229-251

  16. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics

    • Michael W. Riggs, Deborah A. Schaefer
    Pages 253-282

  17. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

    • Jennifer L. Murphy, Michael J. Arrowood
    Pages 283-302

  18. High-Content Screening for Cryptosporidium Drug Discovery

    • Melissa S. Love, Case W. McNamara
    Pages 303-317

  19. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR

    • Haili Zhang, Guan Zhu
    Pages 319-334

  20. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds

    • Nigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, Samuel Arnold
    Pages 335-350
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Keywords

About this book

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.


The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Atlanta Veterans Affairs Medical Center, Decatur, USA

    Jan R. Mead

  • Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA

    Michael J. Arrowood

Bibliographic Information

  • Book Title: Cryptosporidium

  • Book Subtitle: Methods and Protocols

  • Editors: Jan R. Mead, Michael J. Arrowood

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9748-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9747-3Published: 27 August 2019

  • Softcover ISBN: 978-1-4939-9750-3Published: 27 August 2020

  • eBook ISBN: 978-1-4939-9748-0Published: 26 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 407

  • Number of Illustrations: 22 b/w illustrations, 71 illustrations in colour

  • Topics: Parasitology, Microbiology, Infectious Diseases

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